Herpes simplex virus co-infection facilitates rolling circle replication of the adeno-associated virus genome.
Journal
PLoS pathogens
ISSN: 1553-7374
Titre abrégé: PLoS Pathog
Pays: United States
ID NLM: 101238921
Informations de publication
Date de publication:
06 2021
06 2021
Historique:
received:
04
03
2021
accepted:
13
05
2021
revised:
11
06
2021
pubmed:
2
6
2021
medline:
8
10
2021
entrez:
1
6
2021
Statut:
epublish
Résumé
Adeno-associated virus (AAV) genome replication only occurs in the presence of a co-infecting helper virus such as adenovirus type 5 (AdV5) or herpes simplex virus type 1 (HSV-1). AdV5-supported replication of the AAV genome has been described to occur in a strand-displacement rolling hairpin replication (RHR) mechanism initiated at the AAV 3' inverted terminal repeat (ITR) end. It has been assumed that the same mechanism applies to HSV-1-supported AAV genome replication. Using Southern analysis and nanopore sequencing as a novel, high-throughput approach to study viral genome replication we demonstrate the formation of double-stranded head-to-tail concatemers of AAV genomes in the presence of HSV-1, thus providing evidence for an unequivocal rolling circle replication (RCR) mechanism. This stands in contrast to the textbook model of AAV genome replication when HSV-1 is the helper virus.
Identifiants
pubmed: 34061891
doi: 10.1371/journal.ppat.1009638
pii: PPATHOGENS-D-21-00480
pmc: PMC8195378
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1009638Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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