Contemporary Transposon Tools: A Review and Guide through Mechanisms and Applications of
gene therapy
genetic screens
genome engineering
induced pluripotent stem cells
nonviral
transgenesis
transposition
transposon
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
11 May 2021
11 May 2021
Historique:
received:
16
04
2021
revised:
30
04
2021
accepted:
05
05
2021
entrez:
2
6
2021
pubmed:
3
6
2021
medline:
10
6
2021
Statut:
epublish
Résumé
Transposons are mobile genetic elements evolved to execute highly efficient integration of their genes into the genomes of their host cells. These natural DNA transfer vehicles have been harnessed as experimental tools for stably introducing a wide variety of foreign DNA sequences, including selectable marker genes, reporters, shRNA expression cassettes, mutagenic gene trap cassettes, and therapeutic gene constructs into the genomes of target cells in a regulated and highly efficient manner. Given that transposon components are typically supplied as naked nucleic acids (DNA and RNA) or recombinant protein, their use is simple, safe, and economically competitive. Thus, transposons enable several avenues for genome manipulations in vertebrates, including transgenesis for the generation of transgenic cells in tissue culture comprising the generation of pluripotent stem cells, the production of germline-transgenic animals for basic and applied research, forward genetic screens for functional gene annotation in model species and therapy of genetic disorders in humans. This review describes the molecular mechanisms involved in transposition reactions of the three most widely used transposon systems currently available (
Identifiants
pubmed: 34064900
pii: ijms22105084
doi: 10.3390/ijms22105084
pmc: PMC8151067
pii:
doi:
Substances chimiques
DNA Transposable Elements
0
DNA-Binding Proteins
0
Transposases
EC 2.7.7.-
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
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