Osteogenic Potential of Mesenchymal Stem Cells from Adipose Tissue, Bone Marrow and Hair Follicle Outer Root Sheath in a 3D Crosslinked Gelatin-Based Hydrogel.
Adipose Tissue
/ metabolism
Alkaline Phosphatase
/ metabolism
Animals
Bone Marrow
/ metabolism
Bone Marrow Cells
/ metabolism
Bone Regeneration
/ physiology
Calcium
/ metabolism
Cell Differentiation
/ physiology
Cell Survival
/ physiology
Cells, Cultured
Gelatin
/ chemistry
Gene Expression
/ physiology
Hair Follicle
/ metabolism
Humans
Hydrogels
/ chemistry
Mesenchymal Stem Cells
/ metabolism
Models, Animal
Osteogenesis
/ physiology
Swine
Tissue Scaffolds
/ chemistry
3D injectable hydrogel
differentiation capacity towards osteogenic lineage
gelatin-based cross-linked hydrogel
hair follicle outer root sheath
mesenchymal stem cells
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
20 May 2021
20 May 2021
Historique:
received:
31
03
2021
revised:
06
05
2021
accepted:
14
05
2021
entrez:
2
6
2021
pubmed:
3
6
2021
medline:
22
6
2021
Statut:
epublish
Résumé
Bone transplantation is regarded as the preferred therapy to treat a variety of bone defects. Autologous bone tissue is often lacking at the source, and the mesenchymal stem cells (MSCs) responsible for bone repair mechanisms are extracted by invasive procedures. This study explores the potential of autologous mesenchymal stem cells derived from the hair follicle outer root sheath (MSCORS). We demonstrated that MSCORS have a remarkable capacity to differentiate in vitro towards the osteogenic lineage. Indeed, when combined with a novel gelatin-based hydrogel called Osteogel, they provided additional osteoinductive cues in vitro that may pave the way for future application in bone regeneration. MSCORS were also compared to MSCs from adipose tissue (ADMSC) and bone marrow (BMMSC) in a 3D Osteogel model. We analyzed gel plasticity, cell phenotype, cell viability, and differentiation capacity towards the osteogenic lineage by measuring alkaline phosphatase (ALP) activity, calcium deposition, and specific gene expression. The novel injectable hydrogel filled an irregularly shaped lesion in a porcine wound model displaying high plasticity. MSCORS in Osteogel showed a higher osteo-commitment in terms of calcium deposition and expression dynamics of
Identifiants
pubmed: 34065598
pii: ijms22105404
doi: 10.3390/ijms22105404
pmc: PMC8161179
pii:
doi:
Substances chimiques
Hydrogels
0
Gelatin
9000-70-8
Alkaline Phosphatase
EC 3.1.3.1
Calcium
SY7Q814VUP
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Department for Oral- and Maxillofacial Surgery, University Hospital Leipzig
ID : Chair to BL
Organisme : Saxon Ministry of Science and Fine Arts (SMWK)
ID : to SIKT
Organisme : German Research Council (DFG)
ID : SFB TRR 67 B12
Organisme : German Research Council (DFG)
ID : SFB TRR 67 B3
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