Citrate Mediated Europium-Based Detection of Oxytetracycline in Citrus Tissues.
Huanglongbing
antibiotic
citrate
citrus
europium
oxytetracycline
Journal
Antibiotics (Basel, Switzerland)
ISSN: 2079-6382
Titre abrégé: Antibiotics (Basel)
Pays: Switzerland
ID NLM: 101637404
Informations de publication
Date de publication:
12 May 2021
12 May 2021
Historique:
received:
02
04
2021
revised:
30
04
2021
accepted:
09
05
2021
entrez:
2
6
2021
pubmed:
3
6
2021
medline:
3
6
2021
Statut:
epublish
Résumé
Oxytetracycline (OTC) and streptomycin have been used for the control of several plant diseases and were recently permitted for the control of citrus greening disease, Huanglongbing. Consequently, sensitive and reliable methods are highly needed for the detection of OTC in citrus tissues. Herein, we studied the replacement of cetyltrimethylammonium chloride (CTAC) by citrate (Cit) as a sensitizing agent for the analysis of OTC in citrus tissues using the recently established europium (Eu) method. In addition, we determined the optimal conditions for the formation of the Eu-OTC-Cit ternary complex in tris buffer. Our results showed that the plant matrix significantly decreased the fluorescence intensity of the Eu-OTC-Cit complex even after the replacement of CTAC. Our investigations showed that phenols such as gallic acid degrade slowly at high pH and their degradation was enhanced in the presence of the (Eu
Identifiants
pubmed: 34065819
pii: antibiotics10050566
doi: 10.3390/antibiotics10050566
pmc: PMC8151757
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : National Institute of Food and Agriculture
ID : 2019-70016-29096
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