Epigenetic Changes in Equine Embryos after Short-Term Storage at Different Temperatures.

development embryo embryo-maternal recognition equine methylation transport

Journal

Animals : an open access journal from MDPI
ISSN: 2076-2615
Titre abrégé: Animals (Basel)
Pays: Switzerland
ID NLM: 101635614

Informations de publication

Date de publication:
06 May 2021
Historique:
received: 16 03 2021
revised: 10 04 2021
accepted: 03 05 2021
entrez: 2 6 2021
pubmed: 3 6 2021
medline: 3 6 2021
Statut: epublish

Résumé

In embryos subjected to assisted reproductive techniques, epigenetic modifications may occur that can influence embryonic development and the establishment of pregnancy. In horses, the storage temperature during transport of fresh embryos before transfer is a major concern. The aim of this study was, therefore, to determine the effects of two storage temperatures (5 °C and 20 °C) on equine embryos, collected at day seven after ovulation and stored for 24 h, on: (i) morphological development; (ii) expression of candidate genes associated with embryo growth and development, maternal recognition of pregnancy, methylation and apoptosis, and (iii) gene-specific and global DNA methylation. Embryos (

Identifiants

pubmed: 34066466
pii: ani11051325
doi: 10.3390/ani11051325
pmc: PMC8148113
pii:
doi:

Types de publication

Journal Article

Langues

eng

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Auteurs

Gustavo D A Gastal (GDA)

Center for Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, University of Veterinary Medicine Vienna, 1210 Vienna, Austria.
Instituto Nacional de Investigación Agropecuaria INIA, Estación Experimental La Estanzuela, Ruta 50 km 11, 39173 Colonia, Uruguay.

Dragos Scarlet (D)

Division of Obstetrics, Gynecology and Andrology, Department for Small Animals and Horses, University of Veterinary Medicine Vienna, 1210 Vienna, Austria.

Maria Melchert (M)

Center for Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, University of Veterinary Medicine Vienna, 1210 Vienna, Austria.

Reinhard Ertl (R)

Vetcore Facility, University of Veterinary Medicine Vienna, 1210 Vienna, Austria.

Christine Aurich (C)

Center for Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, University of Veterinary Medicine Vienna, 1210 Vienna, Austria.

Classifications MeSH