The Impact of Fixation on the Detection of Oligodendrocyte Precursor Cell Morphology and Vascular Associations.
NG2
OPC
PDGFRα
blood vessel
fixation
immunofluorescence
protrusion
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
24 05 2021
24 05 2021
Historique:
received:
08
02
2021
revised:
10
05
2021
accepted:
19
05
2021
entrez:
2
6
2021
pubmed:
3
6
2021
medline:
28
10
2021
Statut:
epublish
Résumé
Oligodendrocyte precursor cells (OPCs) display numerous protrusions that extend into the surrounding parenchyma in the brain. Depending on the preparation of the tissue analyzed, these protrusions are more or less visible. We applied six different fixation methods and compared the effect of prolonged and stronger fixation on fluorescence intensity of platelet-derived growth factor receptor alpha, a surface marker of OPCs. Importantly, the fluorescence signal is mostly lost on protrusions as compared to the cell body, which has to be considered for specific analyses. Additionally, we show numerous contacts established between OPCs and the brain vasculature, which will contribute to the understanding of the interactions between these two elements.
Identifiants
pubmed: 34073801
pii: cells10061302
doi: 10.3390/cells10061302
pmc: PMC8225113
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NINDS NIH HHS
ID : R01 NS073425
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS116182
Pays : United States
Organisme : NIH HHS
ID : S10 OD016435
Pays : United States
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