Synthesis and Cellular Labeling of Multifunctional Phosphatidylinositol Bis- and Trisphosphate Derivatives.
caged compounds
cell imaging
lipid transport
phosphoinositides
photo-crosslinking
Journal
Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543
Informations de publication
Date de publication:
01 09 2021
01 09 2021
Historique:
revised:
07
05
2021
received:
12
03
2021
pubmed:
3
6
2021
medline:
4
11
2021
entrez:
2
6
2021
Statut:
ppublish
Résumé
We synthesized the first multifunctionalized phosphoinositide polyphosphate derivatives featuring a photo-removable protecting group ("cage"), a photo-crosslinkable diazirine group, and a terminal alkyne group useful for click chemistry. We demonstrate that the lipid derivatives readily enter cells. After photo-crosslinking, cell fixation and fluorescent tagging via click chemistry, we determined the intracellular location of the lipid derivatives before and after uncaging of the lipids. We find that there is rapid trafficking of PI(3,4)P
Identifiants
pubmed: 34075669
doi: 10.1002/anie.202103599
pmc: PMC8390440
mid: NIHMS1716052
doi:
Substances chimiques
Phosphatidylinositols
0
Polyphosphates
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
19759-19765Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM127631
Pays : United States
Informations de copyright
© 2021 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.
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