Lipid profile of bovine grade-1 blastocysts produced either in vivo or in vitro before and after slow freezing process.
Animals
Blastocyst
/ chemistry
Cattle
Cryopreservation
/ methods
Embryo Culture Techniques
Female
Fertilization in Vitro
Glycerophospholipids
/ isolation & purification
Lipidomics
/ methods
Lysophosphatidylcholines
/ isolation & purification
Male
Oxidation-Reduction
Principal Component Analysis
Sex Factors
Triglycerides
/ isolation & purification
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
02 06 2021
02 06 2021
Historique:
received:
28
01
2021
accepted:
17
05
2021
entrez:
3
6
2021
pubmed:
4
6
2021
medline:
6
11
2021
Statut:
epublish
Résumé
Currently, in vitro embryo production (IVP) is successfully commercially applied in cattle. However, the high sensitivity of embryos to cryopreservation in comparison to in vivo (IVD) embryos slows the dissemination of this biotechnology. Reduced cryotolerance is frequently associated with lipid accumulation in the cytoplasm mainly due to in vitro culture conditions. The objective of this study was to evaluate the lipid composition of biopsied and sexed embryos, produced either in vivo or in vitro from the same Holstein heifers before and after a slow freezing protocol. Lipid extracts were analysed by liquid chromatography-high resolution mass spectrometry, which enabled the detection of 496 features. Our results highlighted a lipid enrichment of IVP embryos in triglycerides and oxidised glycerophospholipids and a reduced abundance in glycerophospholipids. The slow freezing process affected the lipid profiles of IVP and IVD embryos similarly. Lysophosphatidylcholine content was reduced when embryos were frozen/thawed. In conclusion, the embryonic lipid profile is impacted by IVP and slow freezing protocols but not by sex. Lysophosphatidylcholine seemed highly sensitive to cryopreservation and might contribute to explain the lower quality of frozen embryos. Further studies are required to improve embryo freezability by modulating the lipidome.
Identifiants
pubmed: 34078963
doi: 10.1038/s41598-021-90870-8
pii: 10.1038/s41598-021-90870-8
pmc: PMC8172931
doi:
Substances chimiques
Glycerophospholipids
0
Lysophosphatidylcholines
0
Triglycerides
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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