Closing Clostridium botulinum Group III Genomes Using Long-Read Sequencing.


Journal

Microbiology resource announcements
ISSN: 2576-098X
Titre abrégé: Microbiol Resour Announc
Pays: United States
ID NLM: 101728794

Informations de publication

Date de publication:
03 Jun 2021
Historique:
entrez: 3 6 2021
pubmed: 4 6 2021
medline: 4 6 2021
Statut: ppublish

Résumé

Clostridium botulinum group III is the anaerobic Gram-positive bacterium producing the deadly neurotoxin responsible for animal botulism. Here, we used long-read sequencing to produce four complete genomes from Clostridium botulinum group III neurotoxin types C, D, C/D, and D/C. The protocol for obtaining high-molecular-weight DNA from C. botulinum group III is described.

Identifiants

pubmed: 34080898
doi: 10.1128/MRA.01364-20
pmc: PMC8354527
doi:

Types de publication

Journal Article

Langues

eng

Pagination

e0136420

Subventions

Organisme : EC | H2020 | H2020 Priority Excellent Science | H2020 European Research Council (ERC)
ID : 683099
Organisme : Animal Health and Welfare ERA-Net (ANIHWA)
ID : ANIBOTNET

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Auteurs

Cedric Woudstra (C)

Department of Food Hygiene and Environmental Health, University of Helsinki, Helsinki, Finland.

Tommi Mäklin (T)

Department of Mathematics and Statistics, University of Helsinki, Helsinki, Finland.

Yagmur Derman (Y)

Department of Food Hygiene and Environmental Health, University of Helsinki, Helsinki, Finland.

Luca Bano (L)

Microbiology Laboratory, Istituto Zooprofiilattico Sperimentale delle Venezie, Villorba, Italy.

Hanna Skarin (H)

National Veterinary Institute, Uppsala, Sweden.

Christelle Mazuet (C)

National Reference Center for Anaerobic Bacteria and Botulism, Institut Pasteur, Paris, France.

Antti Honkela (A)

Department of Computer Science, University of Helsinki, Helsinki, Finland.

Miia Lindström (M)

Department of Food Hygiene and Environmental Health, University of Helsinki, Helsinki, Finland.

Classifications MeSH