Flow cytometry data mining by cytoChain identifies determinants of exhaustion and stemness in TCR-engineered T cells.
COVID-19
/ blood
Cytokines
/ metabolism
Data Mining
/ methods
Flow Cytometry
/ methods
Genetic Engineering
Humans
Immunologic Memory
Immunophenotyping
Immunotherapy, Adoptive
Receptors, Antigen, T-Cell
/ genetics
Receptors, Chimeric Antigen
/ genetics
SARS-CoV-2
/ immunology
T-Lymphocytes
/ immunology
Adoptive T-cell therapy
COVID-19
Flow Cytometry
High-dimensional analysis
Immune exhaustion
Journal
European journal of immunology
ISSN: 1521-4141
Titre abrégé: Eur J Immunol
Pays: Germany
ID NLM: 1273201
Informations de publication
Date de publication:
08 2021
08 2021
Historique:
revised:
09
04
2021
received:
03
12
2020
accepted:
31
05
2021
pubmed:
4
6
2021
medline:
17
8
2021
entrez:
3
6
2021
Statut:
ppublish
Résumé
The phenotype of infused cells is a major determinant of Adoptive T-cell therapy (ACT) efficacy. Yet, the difficulty in deciphering multiparametric cytometry data limited the fine characterization of cellular products. To allow the analysis of dynamic and complex flow cytometry samples, we developed cytoChain, a novel dataset mining tool and a new analytical workflow. CytoChain was challenged to compare state-of-the-art and innovative culture conditions to generate stem-like memory cells (T
Identifiants
pubmed: 34081326
doi: 10.1002/eji.202049103
doi:
Substances chimiques
Cytokines
0
Receptors, Antigen, T-Cell
0
Receptors, Chimeric Antigen
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1992-2005Subventions
Organisme : European Hematology association
Organisme : Ministero della salute
ID : RF-COVID-19
Organisme : European Commission
ID : T2Evolve
Organisme : Associazione Italiana per al Ricerca sul Cancro (AIRC)
ID : Ig_18458
Organisme : AIRC 5 per Mille
ID : 22737
Organisme : Italian Ministry of Research and University
ID : PRIN_2017WC8499
Organisme : Ministero della Salute
ID : Ricerca Finalizzata_GR-2016-02364847
Informations de copyright
© 2021 Wiley-VCH GmbH.
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