Exosome Transfer Between Pancreatic-cancer Cells Is Associated With Metastasis in a Nude-mouse Model.
Pancreatic cancer
color-coded imaging
exosome transfer
green fluorescent protein
metastasis
nude-mouse
red fluorescent protein
Journal
Anticancer research
ISSN: 1791-7530
Titre abrégé: Anticancer Res
Pays: Greece
ID NLM: 8102988
Informations de publication
Date de publication:
Jun 2021
Jun 2021
Historique:
received:
21
04
2021
revised:
06
05
2021
accepted:
07
05
2021
entrez:
4
6
2021
pubmed:
5
6
2021
medline:
22
6
2021
Statut:
ppublish
Résumé
Cancer-derived exosomes play an important role in metastasis. In the present study, we determined whether exosome transfer between cancer cells is associated with metastasis in a mouse model. AsPC-1 human pancreatic-cancer cells expressing red fluorescent protein (RFP) and AsPC-1 human pancreatic-cancer cells transduced by exosome-specific pCT-CD63-green fluorescent protein (GFP), were co-injected into the spleen of nude mice. Both pancreatic-cancer cell lines grew in the spleen and metastasized to the liver, peritoneum, and lungs, as shown by color-coded imaging. The ratio of GFP-expressing exosomes incorporated in RFP-labeled AsPC-1 cells was statistically-significantly higher in the liver, lung, and peritoneal metastases than in the spleen. Exosome transfer between cancer cells is associated with metastasis. Exosome transfer may play a role in increasing the metastatic capability of the recipient cells.
Sections du résumé
BACKGROUND/AIM
OBJECTIVE
Cancer-derived exosomes play an important role in metastasis. In the present study, we determined whether exosome transfer between cancer cells is associated with metastasis in a mouse model.
MATERIALS AND METHODS
METHODS
AsPC-1 human pancreatic-cancer cells expressing red fluorescent protein (RFP) and AsPC-1 human pancreatic-cancer cells transduced by exosome-specific pCT-CD63-green fluorescent protein (GFP), were co-injected into the spleen of nude mice.
RESULTS
RESULTS
Both pancreatic-cancer cell lines grew in the spleen and metastasized to the liver, peritoneum, and lungs, as shown by color-coded imaging. The ratio of GFP-expressing exosomes incorporated in RFP-labeled AsPC-1 cells was statistically-significantly higher in the liver, lung, and peritoneal metastases than in the spleen.
CONCLUSION
CONCLUSIONS
Exosome transfer between cancer cells is associated with metastasis. Exosome transfer may play a role in increasing the metastatic capability of the recipient cells.
Identifiants
pubmed: 34083272
pii: 41/6/2829
doi: 10.21873/anticanres.15063
doi:
Substances chimiques
Luminescent Proteins
0
Green Fluorescent Proteins
147336-22-9
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2829-2834Informations de copyright
Copyright © 2021 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.