Structure and function at the lipid-protein interface of a pentameric ligand-gated ion channel.
Cys-loop receptors
cardiolipin
cryo-EM
nicotinic receptors
styrene–maleic acid nanodiscs
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
08 06 2021
08 06 2021
Historique:
entrez:
4
6
2021
pubmed:
5
6
2021
medline:
1
12
2021
Statut:
ppublish
Résumé
Although it has long been proposed that membrane proteins may contain tightly bound lipids, their identity, the structure of their binding sites, and their functional and structural relevance have remained elusive. To some extent, this is because tightly bound lipids are often located at the periphery of proteins, where the quality of density maps is usually poorer, and because they may be outcompeted by detergent molecules used during standard purification procedures. As a step toward characterizing natively bound lipids in the superfamily of pentameric ligand-gated ion channels (pLGICs), we applied single-particle cryogenic electron microscopy to fragments of native membrane obtained in the complete absence of detergent-solubilization steps. Because of the heterogeneous lipid composition of membranes in the secretory pathway of eukaryotic cells, we chose to study a bacterial pLGIC (ELIC) expressed in
Identifiants
pubmed: 34083441
pii: 2100164118
doi: 10.1073/pnas.2100164118
pmc: PMC8201805
pii:
doi:
Substances chimiques
Bacterial Proteins
0
Ligand-Gated Ion Channels
0
Lipid Bilayers
0
Recombinant Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NINDS NIH HHS
ID : R01 NS042169
Pays : United States
Organisme : NINDS NIH HHS
ID : R56 NS042169
Pays : United States
Organisme : NIGMS NIH HHS
ID : U24 GM129539
Pays : United States
Déclaration de conflit d'intérêts
The authors declare no competing interest.
Références
Chem Biol. 2001 Feb;8(2):147-55
pubmed: 11251289
Biochem Biophys Res Commun. 1980 Mar 13;93(1):127-33
pubmed: 7378076
Nat Commun. 2020 Jan 2;11(1):55
pubmed: 31896756
Nat Chem Biol. 2019 Dec;15(12):1156-1164
pubmed: 31591563
Chem Phys Lipids. 2019 Jan;218:73-84
pubmed: 30508515
Proc Natl Acad Sci U S A. 2020 Jun 16;117(24):13437-13446
pubmed: 32482881
Nat Methods. 2020 Dec;17(12):1214-1221
pubmed: 33257830
J Struct Biol. 2005 Jul;151(1):41-60
pubmed: 15890530
Nature. 2014 Aug 21;512(7514):333-7
pubmed: 25143115
Nat Commun. 2020 Jul 27;11(1):3752
pubmed: 32719334
Neuron. 2020 Jun 17;106(6):952-962.e5
pubmed: 32275860
Nature. 2020 Nov;587(7832):152-156
pubmed: 33087931
Cell. 2021 Feb 18;184(4):957-968.e21
pubmed: 33567265
Biochem Biophys Res Commun. 1980 Mar 28;93(2):409-14
pubmed: 7190011
Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):14706-11
pubmed: 10611277
Cell Death Differ. 2004 Oct;11(10):1133-45
pubmed: 15181455
Proc Natl Acad Sci U S A. 2002 Aug 20;99(17):11055-60
pubmed: 12167672
J Mol Graph. 1996 Feb;14(1):33-8, 27-8
pubmed: 8744570
Biochemistry. 1986 Feb 25;25(4):830-40
pubmed: 3008814
Nat Methods. 2017 Mar;14(3):290-296
pubmed: 28165473
Sci Adv. 2020 Aug 28;6(35):eabb0780
pubmed: 32923632
Structure. 2018 Feb 6;26(2):337-344.e4
pubmed: 29395788
Methods Enzymol. 2009;464:211-31
pubmed: 19903557
Nat Rev Mol Cell Biol. 2008 Feb;9(2):112-24
pubmed: 18216768
Proc Natl Acad Sci U S A. 2012 Apr 17;109(16):6331-6
pubmed: 22474383
Nucleic Acids Res. 2007 Jul;35(Web Server issue):W375-83
pubmed: 17452350
Proc Natl Acad Sci U S A. 2013 Jun 11;110(24):9710-5
pubmed: 23716676
Nat Methods. 2015 Oct;12(10):943-6
pubmed: 26280328
Elife. 2017 Oct 23;6:
pubmed: 29058676
J Mol Biol. 2003 Oct 31;333(4):721-45
pubmed: 14568533
Biochemistry. 1979 Jan 9;18(1):172-9
pubmed: 420771
Clin Exp Immunol. 2000 Nov;122(2):277-84
pubmed: 11091286
Elife. 2017 Nov 22;6:
pubmed: 29165243
J Biol Chem. 1984 Jul 25;259(14):9188-98
pubmed: 6746645
Biophys J. 2008 Mar 1;94(5):1689-98
pubmed: 18024500
Nature. 2008 Mar 20;452(7185):375-9
pubmed: 18322461
Proc Natl Acad Sci U S A. 2020 Jul 14;117(28):16383-16390
pubmed: 32601238
Biochim Biophys Acta. 2004 Nov 3;1666(1-2):2-18
pubmed: 15519305
FEMS Microbiol Rev. 2016 Jan;40(1):133-59
pubmed: 25862689
Mol Pharmacol. 1974 Nov;10(6):904-32
pubmed: 25771543
Nature. 2003 Jun 26;423(6943):949-55
pubmed: 12827192
Protein Sci. 2021 Jan;30(1):70-82
pubmed: 32881101
J Mol Biol. 2010 Nov 12;403(5):693-705
pubmed: 20863833
Biochim Biophys Acta. 2009 Jun;1787(6):609-16
pubmed: 19254687
Biochim Biophys Acta. 1975 Mar 24;380(3):357-69
pubmed: 1169965
Nat Struct Mol Biol. 2020 Jan;27(1):78-83
pubmed: 31907454
J Struct Biol. 2016 Jul;195(1):93-9
pubmed: 27108186
Nat Struct Mol Biol. 2020 Oct;27(10):892-900
pubmed: 32747785
Bioinformatics. 2020 Feb 1;36(3):765-772
pubmed: 31504163
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
pubmed: 20383002
Biochim Biophys Acta. 1967 Oct 2;144(2):382-90
pubmed: 4863608
Ultramicroscopy. 2013 Dec;135:24-35
pubmed: 23872039
Acta Crystallogr D Biol Crystallogr. 2008 Jan;64(Pt 1):125-32
pubmed: 18094476
Elife. 2019 Nov 14;8:
pubmed: 31724949
Proc Natl Acad Sci U S A. 2020 Jan 21;117(3):1788-1798
pubmed: 31911476
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21
pubmed: 20124702
J Bacteriol. 1988 Feb;170(2):775-80
pubmed: 2828323
Pflugers Arch. 1988 Jan;411(1):94-100
pubmed: 2451217
J Gen Physiol. 2009 Feb;133(2):151-69
pubmed: 19171769
Annu Rev Microbiol. 2003;57:203-24
pubmed: 14527277
Acta Crystallogr D Struct Biol. 2018 Sep 1;74(Pt 9):814-840
pubmed: 30198894
Nature. 2009 Jan 1;457(7225):111-4
pubmed: 18987633
EMBO J. 2007 Mar 21;26(6):1713-25
pubmed: 17332748
Trends Biochem Sci. 2001 Feb;26(2):106-12
pubmed: 11166568
IUCrJ. 2020 Jul 28;7(Pt 5):852-859
pubmed: 32939277
FEBS Lett. 1972 Nov 15;28(1):101-6
pubmed: 4646865
PLoS One. 2013 Sep 13;8(9):e73040
pubmed: 24058458
Biochim Biophys Acta Biomembr. 2020 Sep 1;1862(9):183304
pubmed: 32311340
Nature. 2011 Jun 2;474(7349):54-60
pubmed: 21572436
Biochim Biophys Acta. 2003 May 2;1612(1):1-40
pubmed: 12729927
FEBS Lett. 1980 Dec 29;122(2):193-6
pubmed: 7202709
J Gen Physiol. 2006 Nov;128(5):615-27
pubmed: 17074980
Proc Natl Acad Sci U S A. 2012 Oct 9;109(41):16504-9
pubmed: 22988102