Optimizing Cell Synchronization Using Nocodazole or Double Thymidine Block.
Cell cycle
G1/S
G2/M
Nocodazole
Suspension cells
Synchronization
Thymidine
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
4
6
2021
pubmed:
5
6
2021
medline:
25
8
2021
Statut:
ppublish
Résumé
Cell synchronization is crucial when studying events that take place at specific points of the cell cycle. Several chemical agents can be used to achieve the cell culture synchronization but not all type of cells respond equally to a given concentration of these drugs. Here we describe a simple optimization method to select concentrations and timings for nocodazole or thymidine treatments using fluorescence staining. In addition, we provide detailed protocols to arrest an asynchronous culture of either suspension or adherent cells in G
Identifiants
pubmed: 34085219
doi: 10.1007/978-1-0716-1538-6_9
doi:
Substances chimiques
Fluorescent Dyes
0
Nocodazole
SH1WY3R615
Thymidine
VC2W18DGKR
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
111-121Références
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