Mitochondrial dynamics and degradation in the oleaginous yeast Lipomyces starkeyi.
autophagy
lipid droplet
mitochondria
oleaginous yeast
Journal
Genes to cells : devoted to molecular & cellular mechanisms
ISSN: 1365-2443
Titre abrégé: Genes Cells
Pays: England
ID NLM: 9607379
Informations de publication
Date de publication:
Aug 2021
Aug 2021
Historique:
revised:
25
05
2021
received:
11
05
2021
accepted:
01
06
2021
pubmed:
5
6
2021
medline:
2
9
2021
entrez:
4
6
2021
Statut:
ppublish
Résumé
Emerging evidence implicates the vital role of mitochondria in lipid consumption and storage, highlighting the intimate link between energy production and saving. Although formation of giant lipid droplets, which is the key hallmark of the oleaginous yeast Lipomyces starkeyi, appears to be regulated in response to changes in mitochondrial shape and metabolism, technical limitations of genetic manipulation have become an obstacle to uncover the mitochondrial behavior in this nonconventional yeast. Here, we established an L. starkeyi strain stably expressing a fluorescent marker for monitoring mitochondrial morphology and degradation and found that mitochondria are mostly fragmented in L. starkeyi cells under fermentable, nonfermentable, and nitrogen depletion conditions. Notably, a fraction of mitochondria-specific fluorescent signals was localized to the vacuole, a lytic organelle in yeast, indicating degradation of mitochondria in those cells. This possible catabolic event was more predominant in cells under nutrient-poor conditions than that in cells under nutrient-rich conditions, concomitantly with lipid droplet formation. Collectively, our studies provide a new tool to investigate mitochondrial dynamics in L. starkeyi and decipher the potential role of mitochondrial degradation in lipid metabolism.
Substances chimiques
Nitrogen
N762921K75
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
627-635Subventions
Organisme : Osaka University
ID : International Joint Research Promotion Programs
Organisme : Japan Society for the Promotion of Science
ID : Scientific Research (B) / 19H03222
Organisme : Japan Society for the Promotion of Science
ID : Scientific Research on Innovative Areas / 20H05324
Informations de copyright
© 2021 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.
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