Generation of three human iPSC lines from patients with a spontaneous late-onset Alzheimer's disease and three sex- and age-matched healthy controls.

Alzheimer Disease Cell Differentiation Cellular Reprogramming Germ Layers Humans Induced Pluripotent Stem Cells Leukocytes, Mononuclear

Journal

Stem cell research
ISSN: 1876-7753
Titre abrégé: Stem Cell Res
Pays: England
ID NLM: 101316957

Informations de publication

Date de publication:
05 2021
Historique:
received: 22 01 2021
revised: 12 04 2021
accepted: 25 04 2021
entrez: 5 6 2021
pubmed: 6 6 2021
medline: 1 7 2021
Statut: ppublish

Résumé

Human induced pluripotent stem cell (iPSC) lines were generated from patients with spontaneous late-onset Alzheimer's disease (AD) and three healthy control individuals. Peripheral blood mononuclear cells were reprogrammed with Yamanaka factors (OSKM) using a commercially available Epi5 Reprogramming Kit. The pluripotency of iPSCs was confirmed by the expression of pluripotency factors and by their ability to differentiate to all three germ layers in vitro. Newly derived cell lines can be used to model Alzheimer's disease in vitro.

Identifiants

DOI: 10.1016/j.scr.2021.102378 PMID: 34088007
pubmed: 34088007
pii: S1873-5061(21)00224-5
doi: 10.1016/j.scr.2021.102378
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

102378

Informations de copyright

Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Auteurs

Jan Raska (J)

Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.

Hana Klimova (H)

Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.

Katerina Sheardova (K)

International Clinical Research Center (ICRC) and Neurology Department, St. Anne's University Hospital, Brno, Czech Republic; Neurology Department, St. Anne's University Hospital, Brno, Czech Republic.

Veronika Fedorova (V)

Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.

Hana Hribkova (H)

Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.

Veronika Pospisilova (V)

Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.

Simona Vochyanova (S)

Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.

Tereza Vanova (T)

Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic; International Clinical Research Center (ICRC) and Neurology Department, St. Anne's University Hospital, Brno, Czech Republic.

Dasa Bohaciakova (D)

Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic; International Clinical Research Center (ICRC) and Neurology Department, St. Anne's University Hospital, Brno, Czech Republic. Electronic address: bohaciakova@med.muni.cz.

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Classifications MeSH

questionsmedicales.fr › Troubles mentaux › Troubles neurocognitifs › Démence › Maladie d'Alzheimer › Generation of three human iPSC lines from...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Différenciation cellulaire › Generation of three human iPSC lines from...
questionsmedicales.fr › Phénomènes génétiques › Reprogrammation cellulaire › Generation of three human iPSC lines from...
questionsmedicales.fr › Structures de l'embryon › Feuillets embryonnaires › Generation of three human iPSC lines from...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Generation of three human iPSC lines from...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches pluripotentes › Cellules souches pluripotentes induites › Generation of three human iPSC lines from...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Agranulocytes › Generation of three human iPSC lines from...