Analysis of honey environmental DNA indicates that the honey bee (Apis mellifera L.) trypanosome parasite Lotmaria passim is widespread in the apiaries of the North of Italy.
Health
Monitoring
PCR
Parasite
Trypanosomatidae
Journal
Journal of invertebrate pathology
ISSN: 1096-0805
Titre abrégé: J Invertebr Pathol
Pays: United States
ID NLM: 0014067
Informations de publication
Date de publication:
09 2021
09 2021
Historique:
received:
14
03
2021
revised:
09
05
2021
accepted:
28
05
2021
pubmed:
7
6
2021
medline:
30
11
2021
entrez:
6
6
2021
Statut:
ppublish
Résumé
Lotmaria passim is a trypanosomatid that infects honey bees. In this study, we established an axenic culture of L. passim from Italian isolates and then used its DNA as a control in subsequent analyses that investigated environmental DNA (eDNA) to detect this trypasonosomatid. The source of eDNA was honey, which has been already demonstrated to be useful to detect honey bee parasites. DNA from a total of 164 honey samples collected in the North of Italy was amplified with three L. passim specific PCR primers and 78% of the analysed samples gave positive results. These results indicated a high prevalence rate of this trypanosomatid in the North of Italy, where it might be considered another threat to honey bee health.
Identifiants
pubmed: 34090931
pii: S0022-2011(21)00095-1
doi: 10.1016/j.jip.2021.107628
pii:
doi:
Substances chimiques
DNA, Environmental
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
107628Informations de copyright
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