High-content approaches to anthelmintic drug screening.
anthelmintic
drug screening
high-content imaging
nematode
platyhelminth
Journal
Trends in parasitology
ISSN: 1471-5007
Titre abrégé: Trends Parasitol
Pays: England
ID NLM: 100966034
Informations de publication
Date de publication:
09 2021
09 2021
Historique:
received:
02
03
2021
revised:
03
05
2021
accepted:
11
05
2021
pubmed:
8
6
2021
medline:
2
10
2021
entrez:
7
6
2021
Statut:
ppublish
Résumé
Most anthelmintics were discovered through in vivo screens using animal models of infection. Developing in vitro assays for parasitic worms presents several challenges. The lack of in vitro life cycle culture protocols requires harvesting worms from vertebrate hosts or vectors, limiting assay throughput. Once worms are removed from the host environment, established anthelmintics often show no obvious phenotype - raising concerns about the predictive value of many in vitro assays. However, with recent progress in understanding how anthelmintics subvert host-parasite interactions, and breakthroughs in high-content imaging and machine learning, in vitro assays have the potential to discern subtle cryptic parasite phenotypes. These may prove better endpoints than conventional in vitro viability assays.
Identifiants
pubmed: 34092518
pii: S1471-4922(21)00113-6
doi: 10.1016/j.pt.2021.05.004
pmc: PMC8364491
mid: NIHMS1711456
pii:
doi:
Substances chimiques
Anthelmintics
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
780-789Subventions
Organisme : NIAID NIH HHS
ID : R01 AI151171
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI146540
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI153545
Pays : United States
Informations de copyright
Copyright © 2021 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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