Effect of EPA on Neonatal Pig Sertoli Cells "
Animals
Animals, Newborn
Antineoplastic Combined Chemotherapy Protocols
/ adverse effects
Cancer Survivors
Cells, Cultured
Child
Cisplatin
/ adverse effects
Eicosapentaenoic Acid
/ pharmacology
Fertility
/ drug effects
Fertility Preservation
/ methods
Gonads
/ drug effects
Humans
Male
Sertoli Cells
/ cytology
Swine
Sertoli cells
chemotherapeutic agents
eicosapentaenoic acid
infertility
pre-pubertal boys
Journal
Frontiers in endocrinology
ISSN: 1664-2392
Titre abrégé: Front Endocrinol (Lausanne)
Pays: Switzerland
ID NLM: 101555782
Informations de publication
Date de publication:
2021
2021
Historique:
received:
13
04
2021
accepted:
05
05
2021
entrez:
7
6
2021
pubmed:
8
6
2021
medline:
16
2
2022
Statut:
epublish
Résumé
The incidence of cancer in pre-pubertal boys has significantly increased and, it has been recognized that the gonado-toxic effect of the cancer treatments may lead to infertility. Here, we have evaluated the effects on porcine neonatal Sertoli cells (SCs) of three commonly used chemotherapy drugs; cisplatin, 4-Hydroperoxycyclophosphamide and doxorubicin. All three drugs induced a statistical reduction of 5-hydroxymethylcytosine in comparison with the control group, performed by Immunofluorescence Analysis. The gene and protein expression levels of GDNF, were significantly down-regulated after treatment to all three chemotherapy drugs comparison with the control group. Specifically, differences in the mRNA levels of GDNF were: 0,8200 ± 0,0440, 0,6400 ± 0,0140, 0,4400 ± 0,0130 fold change at 0.33, 1.66, and 3.33μM cisplatin concentrations, respectively (**p < 0.01 at 0.33 and 1.66 μM
Identifiants
pubmed: 34093450
doi: 10.3389/fendo.2021.694796
pmc: PMC8174840
doi:
Substances chimiques
Eicosapentaenoic Acid
AAN7QOV9EA
Cisplatin
Q20Q21Q62J
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
694796Informations de copyright
Copyright © 2021 Arato, Ceccarelli, Mancuso, Bellucci, Lilli, Ferolla, Perruccio, D’Arpino, Aglietti, Calafiore, Cameron, Calvitti, Baroni, Vecchini and Luca.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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