The Vaginal Microbial Signatures of Preterm Birth Delivery in Indian Women.
16S rRNA gene sequencing
Lactobacillus
microbial ecology
preterm birth
vaginal microbiota
Journal
Frontiers in cellular and infection microbiology
ISSN: 2235-2988
Titre abrégé: Front Cell Infect Microbiol
Pays: Switzerland
ID NLM: 101585359
Informations de publication
Date de publication:
2021
2021
Historique:
received:
28
10
2020
accepted:
23
04
2021
entrez:
7
6
2021
pubmed:
8
6
2021
medline:
3
7
2021
Statut:
epublish
Résumé
The incidence of preterm birth (PTB) in India is around 13%. Specific bacterial communities or individual taxon living in the vaginal milieu of pregnant women is a potential risk factor for PTB and may play an important role in its pathophysiology. Besides, bacterial taxa associated with PTB vary across populations. Conduct a comparative analysis of vaginal microbiome composition and microbial genomic repertoires of women who enrolled in the Interdisciplinary Group for Advanced Research on Birth Outcomes - A DBT India Initiative (GARBH-Ini) pregnancy cohort to identify bacterial taxa associated with term birth (TB) and PTB in Indian women. Vaginal swabs were collected during all three trimesters from 38 pregnant Indian women who delivered spontaneous term (n=20) and preterm (n=18) neonates. Paired-end sequencing of V3-V4 region of 16S rRNA gene was performed using the metagenomic DNA isolated from vaginal swabs (n=115). Whole genome sequencing of bacterial species associated with birth outcomes was carried out by shotgun method. Vaginal microbiome of both term and preterm samples reveals similar alpha diversity indices. However, significantly higher abundance of Our findings indicate that the dominance of specific
Sections du résumé
Background
The incidence of preterm birth (PTB) in India is around 13%. Specific bacterial communities or individual taxon living in the vaginal milieu of pregnant women is a potential risk factor for PTB and may play an important role in its pathophysiology. Besides, bacterial taxa associated with PTB vary across populations.
Objective
Conduct a comparative analysis of vaginal microbiome composition and microbial genomic repertoires of women who enrolled in the Interdisciplinary Group for Advanced Research on Birth Outcomes - A DBT India Initiative (GARBH-Ini) pregnancy cohort to identify bacterial taxa associated with term birth (TB) and PTB in Indian women.
Methods
Vaginal swabs were collected during all three trimesters from 38 pregnant Indian women who delivered spontaneous term (n=20) and preterm (n=18) neonates. Paired-end sequencing of V3-V4 region of 16S rRNA gene was performed using the metagenomic DNA isolated from vaginal swabs (n=115). Whole genome sequencing of bacterial species associated with birth outcomes was carried out by shotgun method.
Results
Vaginal microbiome of both term and preterm samples reveals similar alpha diversity indices. However, significantly higher abundance of
Conclusion
Our findings indicate that the dominance of specific
Identifiants
pubmed: 34094994
doi: 10.3389/fcimb.2021.622474
pmc: PMC8169982
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
622474Investigateurs
Shinjini Bhatnagar
(S)
Bhabatosh Das
(B)
Vineeta Bal
(V)
Bapu Koundinya Desiraju
(BK)
Pallavi Kshetrapal
(P)
Sumit Misra
(S)
Uma Chandra Mouli Natchu
(UCM)
Satyajit Rath
(S)
Kanika Sachdeva
(K)
Dharmendra Sharma
(D)
Amanpreet Singh
(A)
Shailaja Sopory
(S)
Ramachandran Thiruvengadam
(R)
Nitya Wadhwa
(N)
Arindam Maitra
(A)
Partha P Majumder
(PP)
Souvik Mukherjee
(S)
Tushar K Maiti
(TK)
Monika Bahl
(M)
Shubra Bansal
(S)
Umesh Mehta
(U)
Sunita Sharma
(S)
Brahmdeep Sindhu
(B)
Sugandha Arya
(S)
Rekha Bharti
(R)
Harish Chellani
(H)
Pratima Mittal
(P)
Anju Garg
(A)
Siddharth Ramji
(S)
Ashok Khurana
(A)
Reva Tripathi
(R)
Yashdeep Gupta
(Y)
Smriti Hari
(S)
Nikhil Tandon
(N)
Rakesh Gupta
(R)
Dinakar M Salunke
(DM)
G Balakrish Nair
(GB)
Informations de copyright
Copyright © 2021 Kumar, Kumari, Talukdar, Kothidar, Sarkar, Mehta, Kshetrapal, Wadhwa, Thiruvengadam, Desiraju, Nair, Bhatnagar, Mukherjee, Das and GARBH-Ini Study Group.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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