An optimized protocol for isolation of S-nitrosylated proteins from

Animals Caenorhabditis elegans / chemistry Caenorhabditis elegans Proteins / analysis Nitrosation Proteome / analysis Proteomics / methods S-Nitrosothiols
Model Organisms Protein Biochemistry Proteomics

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
18 06 2021
Historique:
entrez: 7 6 2021
pubmed: 8 6 2021
medline: 8 6 2021
Statut: epublish

Résumé

Post-translational modification by S-nitrosylation regulates numerous cellular functions and impacts most proteins across phylogeny. We describe a protocol for isolating S-nitrosylated proteins (SNO-proteins) from

Identifiants

DOI: 10.1016/j.xpro.2021.100547 PMID: 34095861 PMC: PMC8164088
pubmed: 34095861
doi: 10.1016/j.xpro.2021.100547
pii: S2666-1667(21)00254-9
pmc: PMC8164088
doi:

Substances chimiques

Caenorhabditis elegans Proteins 0
Proteome 0
S-Nitrosothiols 0

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Pagination

100547

Subventions

Organisme : NIDDK NIH HHS
ID : R01 DK119506
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM099921
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK128347
Pays : United States
Organisme : NHLBI NIH HHS
ID : P01 HL128192
Pays : United States
Organisme : NHLBI NIH HHS
ID : P01 HL075443
Pays : United States

Informations de copyright

© 2021 The Author(s).

Déclaration de conflit d'intérêts

Jonathan Stamler is a co-founder of the company SNO bio, which develops SNO-based therapeutics.

Références

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pubmed: 19483679
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pubmed: 16291226
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pubmed: 23415229
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pubmed: 26494922

Auteurs

Puneet Seth (P)

Institute for Transformative Molecular Medicine and Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA.

Richard T Premont (RT)

Institute for Transformative Molecular Medicine and Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA.
Harrington Discovery Institute, University Hospitals Cleveland Medical Center, Cleveland, OH 44106, USA.

Jonathan S Stamler (JS)

Institute for Transformative Molecular Medicine and Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA.
Harrington Discovery Institute, University Hospitals Cleveland Medical Center, Cleveland, OH 44106, USA.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › An optimized protocol for isolation of...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Helminthes › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Caenorhabditis › Caenorhabditis elegans › An optimized protocol for isolation of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines d'helminthes › Protéines de Caenorhabditis elegans › An optimized protocol for isolation of...
questionsmedicales.fr › Métabolisme › Nitrosation › An optimized protocol for isolation of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéome › An optimized protocol for isolation of...
questionsmedicales.fr › Disciplines des sciences naturelles › Chimie › Biochimie › Protéomique › An optimized protocol for isolation of...
questionsmedicales.fr › Composés chimiques organiques › Composés du soufre › Thiols › S-Nitrosothiols › An optimized protocol for isolation of...