Identification of Circulating

Alzheimer’s Disease MicroRNAse Quantitative Reverse Transcription Polymerase Chain Reaction Serum

Journal

Cell journal
ISSN: 2228-5806
Titre abrégé: Cell J
Pays: Iran
ID NLM: 101566618

Informations de publication

Date de publication:
Jul 2021
Historique:
received: 11 07 2019
accepted: 08 12 2019
entrez: 7 6 2021
pubmed: 8 6 2021
medline: 8 6 2021
Statut: ppublish

Résumé

Alzheimer's disease (AD) is a type of dementia. Currently, there are not any existing and reliable methods for the prognosis or diagnosis of AD. Hence, finding a diagnostic/prognostic biomarker for AD helps physicians to prescribe the treatments and methods preventing disease progression. Circulating microRNAs (miRNAs) are the most promising biomarkers due to their non-invasive and easily accessible for diagnosis and prognosis of AD. The aim of current study is to evaluate expression levels of two unwell-known circulating miRNAs including In this case and control study, to get the gene targets related to these two miRNAs, TargetScan, miRTargetLink Human and mirDIP web servers were applied. In addition, gene networks and gene ontology enrichment analysis were performed by STRING 10.5, KEGG and ShinyGO v0.41. Experimentally, expression levels of these two miRNAs in the serum of 21 patients with AD and 23 healthy individuals were compared using the quantitative reverse transcription polymerase chain reaction (qRT-PCR) method. The pathophysiological pathways associated with these two miRNAs were nucleotide metabolism and cellular response to stress pathway. Furthermore, the upregulated expression levels of Non-significant results were obtained from the expression levels of AD patients and two significant pathways were obtained by networks and gene enrichment analysis.

Identifiants

DOI: 10.22074/cellj.2021.7047 PMID: 34096222 PMC: PMC8181312
pubmed: 34096222
doi: 10.22074/cellj.2021.7047
pmc: PMC8181312
doi:

Types de publication

Journal Article

Langues

eng

Pagination

211-217

Informations de copyright

Copyright© by Royan Institute. All rights reserved.

Déclaration de conflit d'intérêts

There is no conflict of interest in this study.

Références

Ann Neurol. 1991 Oct;30(4):572-80
pubmed: 1789684
Nat Rev Neurosci. 2007 Jul;8(7):499-509
pubmed: 17551515
Alzheimers Res Ther. 2014 Jul 03;6(4):37
pubmed: 25024750
Braz J Med Biol Res. 2007 Nov;40(11):1435-40
pubmed: 17934639
Mech Ageing Dev. 2013 Oct;134(10):427-33
pubmed: 23665461
Mol Psychiatry. 2015 Oct;20(10):1188-96
pubmed: 25349172
Front Cell Neurosci. 2014 Jun 10;8:156
pubmed: 24959119
Neurodegeneration. 1996 Dec;5(4):417-21
pubmed: 9117556
Front Cell Neurosci. 2013 Apr 10;7:38
pubmed: 23596393
Biochem Biophys Res Commun. 2017 Feb 19;483(4):1156-1165
pubmed: 27524239
PLoS One. 2013;8(3):e59011
pubmed: 23527072
Toxicol Sci. 2015 Mar;144(1):7-16
pubmed: 25740792
Biochim Biophys Acta. 2012 May;1822(5):639-49
pubmed: 22037588
J Alzheimers Dis. 2010;20 Suppl 2:S499-512
pubmed: 20413847
Anal Quant Cytol Histol. 1996 Jun;18(3):209-13
pubmed: 8790834
J Neurochem. 2006 Jan;96(1):1-13
pubmed: 16305625
Br J Cancer. 2014 Apr 29;110(9):2291-9
pubmed: 24595006
Alzheimers Dement. 2011 May;7(3):257-62
pubmed: 21514247
Trends Mol Med. 2008 Feb;14(2):45-53
pubmed: 18218341
BMC Biotechnol. 2006 Dec 12;6:47
pubmed: 17164008
PLoS One. 2013 Jun 18;8(6):e66393
pubmed: 23823624
Proc Natl Acad Sci U S A. 2010 Oct 26;107(43):18670-5
pubmed: 20937894
J Psychiatr Res. 2014 Oct;57:65-73
pubmed: 24998397
Dev Neurosci. 1998;20(4-5):291-9
pubmed: 9778565
Mech Ageing Dev. 2012 Nov-Dec;133(11-12):675-85
pubmed: 23041385
J Neurochem. 2007 Aug;102(4):1383-94
pubmed: 17488276
Ageing Res Rev. 2017 May;35:350-363
pubmed: 27903442
Neurology. 1984 Jul;34(7):939-44
pubmed: 6610841
EMBO J. 2008 Oct 8;27(19):2616-27
pubmed: 18756266
Brain Res Brain Res Rev. 1995 Sep;21(2):195-218
pubmed: 8866675
Front Aging Neurosci. 2010 May 21;2:
pubmed: 20725518
Biochim Biophys Acta. 2016 Sep;1862(9):1617-27
pubmed: 27264337
PLoS One. 2013 Jul 29;8(7):e69807
pubmed: 23922807
J Neuropathol Exp Neurol. 2001 Aug;60(8):759-67
pubmed: 11487050
J Biol Chem. 2011 Dec 2;286(48):41442-41454
pubmed: 21971048
Biochim Biophys Acta. 2011 Dec;1812(12):1630-9
pubmed: 21920438
Neurotherapeutics. 2010 Oct;7(4):399-412
pubmed: 20880504
Eur J Neurosci. 2005 Mar;21(6):1469-77
pubmed: 15845075
Nature. 2004 Aug 5;430(7000):631-9
pubmed: 15295589
Acta Neuropathol. 2011 Feb;121(2):193-205
pubmed: 20936480

Auteurs

Maryam Heydari (M)

Division of Genetics, Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran.

Zohreh Hojati (Z)

Division of Genetics, Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran. Email: z.hojati@sci.ui.ac.ir.

Moein Dehbashi (M)

Division of Genetics, Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran.

Classifications MeSH