Modeling of enzymatic activity of free β-glucosidase from palm weevil, Rhynchophorus palmarum Linn. (Coleoptera: Curculionidae) larvae: Effects of pH and temperature.
Equilibrium model
Kinetic modeling
Rhynchophorus palmarum
β-Glucosidase
Journal
Biophysical chemistry
ISSN: 1873-4200
Titre abrégé: Biophys Chem
Pays: Netherlands
ID NLM: 0403171
Informations de publication
Date de publication:
09 2021
09 2021
Historique:
received:
13
03
2021
revised:
02
05
2021
accepted:
03
05
2021
pubmed:
8
6
2021
medline:
17
12
2021
entrez:
7
6
2021
Statut:
ppublish
Résumé
Palm weevil, Rhynchophorus palmarum L., is an important pest of palm trees (Elaeis guineensis) around the tropical regions. Characterization of their digestive enzymes could be an important stage to develop appropriate pest control strategies. Study of these enzymes could also be of interest in different biotechnological applications. Among digestive enzymes, there is β-glucosidase which hydrolytically catalyzes the β-glycosidic linkage of glycosides. In the present work, the catalytic activity of β-glucosidase in the digestive juice of last larval instar of R. palmarum L. (Rpbgl) has been investigated using p-nitrophenyl-β-D-glucopyranoside (pNPG) as substrate. The "classical" physico-chemical properties for purified Rpbgl have been determined by the help of enzymatic activity modeling. Thus, the values of (325.4 ± 0.2) K, 5.28 ± 0.07 and (37.9 ± 0.6) kJ mol
Identifiants
pubmed: 34098161
pii: S0301-4622(21)00093-4
doi: 10.1016/j.bpc.2021.106611
pii:
doi:
Substances chimiques
beta-Glucosidase
EC 3.2.1.21
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
106611Informations de copyright
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