Taphonomy of experimental burials in Taphos-m: The role of fungi.
Animal model
Anthropology
Antropología
Archaeology
Arqueología
Carcasas
Carcasses
Enterramientos experimentales
Experimental burial
Forensic mycology
Fungi
Hisopos estériles
Hongos
Micología forense
Modelo animal
Placas de contacto RODAC
RODAC contact plates
Sterile swab
Tafonomía
Taphonomy
Journal
Revista iberoamericana de micologia
ISSN: 2173-9188
Titre abrégé: Rev Iberoam Micol
Pays: Spain
ID NLM: 9425531
Informations de publication
Date de publication:
Historique:
received:
18
08
2020
revised:
10
02
2021
accepted:
12
02
2021
pubmed:
9
6
2021
medline:
29
10
2021
entrez:
8
6
2021
Statut:
ppublish
Résumé
The fungi present in the decaying remains enable a better understanding of the processes of decomposition after death. There are not many studies about fungi on decaying bodies and it is not known which fungal sampling methods are effective. The main objective of this study was to find the best method for sampling fungi in carcasses, prove the effectiveness of this method and identify the fungal colonies in animal carcasses from experimental burials. Samples from 13 carcasses of Sus scrofa domestica, from the experimental project Taphos-m, were taken with different materials: spatula, sterile swabs and RODAC contact plates. RODAC contact plates with the RBA culture medium showed higher proliferation of fungal colonies. Thirty genera of fungi were isolated from different substrates (bone, tissue, lime). Most of the fungi genera or groups identified have been described before in the literature, but the substrates they came from were different in some cases. Sampling with RODAC contact plates was found to be the most effective method, as it provides a nutritional culture medium that may allow growth since the moment of sampling. Fungi colonies grew better in RBA culture medium because bacterial growth is inhibited. Most of the observed fungi are related to the environment but some others have been found related to decomposing bodies for the first time.
Sections du résumé
BACKGROUND
BACKGROUND
The fungi present in the decaying remains enable a better understanding of the processes of decomposition after death. There are not many studies about fungi on decaying bodies and it is not known which fungal sampling methods are effective.
AIMS
OBJECTIVE
The main objective of this study was to find the best method for sampling fungi in carcasses, prove the effectiveness of this method and identify the fungal colonies in animal carcasses from experimental burials.
METHODS
METHODS
Samples from 13 carcasses of Sus scrofa domestica, from the experimental project Taphos-m, were taken with different materials: spatula, sterile swabs and RODAC contact plates.
RESULTS
RESULTS
RODAC contact plates with the RBA culture medium showed higher proliferation of fungal colonies. Thirty genera of fungi were isolated from different substrates (bone, tissue, lime). Most of the fungi genera or groups identified have been described before in the literature, but the substrates they came from were different in some cases.
CONCLUSIONS
CONCLUSIONS
Sampling with RODAC contact plates was found to be the most effective method, as it provides a nutritional culture medium that may allow growth since the moment of sampling. Fungi colonies grew better in RBA culture medium because bacterial growth is inhibited. Most of the observed fungi are related to the environment but some others have been found related to decomposing bodies for the first time.
Identifiants
pubmed: 34099381
pii: S1130-1406(21)00020-6
doi: 10.1016/j.riam.2021.02.003
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
125-131Informations de copyright
Copyright © 2021 Asociación Española de Micología. Publicado por Elsevier España, S.L.U. All rights reserved.