Human immune system adaptations to simulated microgravity revealed by single-cell mass cytometry.
Adaptation, Physiological
/ genetics
Adult
Flow Cytometry
/ methods
Humans
Immune System
/ cytology
Middle Aged
RNA, Messenger
/ genetics
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction
/ genetics
Single-Cell Analysis
/ methods
Space Flight
/ methods
T-Lymphocytes
/ cytology
Transcriptome
/ immunology
Weightlessness Simulation
Young Adult
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
07 06 2021
07 06 2021
Historique:
received:
17
02
2021
accepted:
11
05
2021
entrez:
8
6
2021
pubmed:
9
6
2021
medline:
9
11
2021
Statut:
epublish
Résumé
Exposure to microgravity (µG) during space flights produces a state of immunosuppression, leading to increased viral shedding, which could interfere with long term missions. However, the cellular mechanisms that underlie the immunosuppressive effects of µG are ill-defined. A deep understanding of human immune adaptations to µG is a necessary first step to design data-driven interventions aimed at preserving astronauts' immune defense during short- and long-term spaceflights. We employed a high-dimensional mass cytometry approach to characterize over 250 cell-specific functional responses in 18 innate and adaptive immune cell subsets exposed to 1G or simulated (s)µG using the Rotating Wall Vessel. A statistically stringent elastic net method produced a multivariate model that accurately stratified immune responses observed in 1G and sµG (p value 2E-4, cross-validation). Aspects of our analysis resonated with prior knowledge of human immune adaptations to µG, including the dampening of Natural Killer, CD4
Identifiants
pubmed: 34099760
doi: 10.1038/s41598-021-90458-2
pii: 10.1038/s41598-021-90458-2
pmc: PMC8184772
doi:
Substances chimiques
RNA, Messenger
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
11872Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM137936
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM138353
Pays : United States
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