Submucosal microbiome of peri-implant sites: A cross-sectional study.
dental implant
dysbiosis
microbiome
next-generation sequencing
Journal
Journal of clinical periodontology
ISSN: 1600-051X
Titre abrégé: J Clin Periodontol
Pays: United States
ID NLM: 0425123
Informations de publication
Date de publication:
09 2021
09 2021
Historique:
revised:
29
04
2021
received:
01
10
2020
accepted:
07
05
2021
pubmed:
9
6
2021
medline:
25
2
2023
entrez:
8
6
2021
Statut:
ppublish
Résumé
To study the peri-implant submucosal microbiome in relation to implant disease status, dentition status, smoking habit, gender, implant location, implant system, time of functional loading, probing pocket depth (PPD), and presence of bleeding on probing. Biofilm samples were collected from the deepest peri-implant site of 41 patients with paper points, and analysed using 16S rRNA gene pyrosequencing. We observed differences in microbial profiles by PPD, implant disease status, and dentition status. Microbiota in deep pockets included higher proportions of the genera Fusobacterium, Prevotella, and Anaeroglobus compared with shallow pockets that harboured more Rothia, Neisseria, Haemophilus, and Streptococcus. Peri-implantitis (PI) sites were dominated by Fusobacterium and Treponema compared with healthy implants and peri-implant mucositis, which were mostly colonized by Rothia and Streptococcus. Partially edentulous (PE) individuals presented more Fusobacterium, Prevotella, and Rothia, whereas fully edentulous individuals presented more Veillonella and Streptococcus. PPD, implant disease status, and dentition status may affect the submucosal ecology leading to variation in composition of the microbiome. Deep pockets, PI, and PE individuals were dominated by Gram-negative anaerobic taxa.
Identifiants
pubmed: 34101220
doi: 10.1111/jcpe.13502
pmc: PMC8457166
doi:
Substances chimiques
Dental Implants
0
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1228-1239Informations de copyright
© 2021 The Authors. Journal of Clinical Periodontology published by John Wiley & Sons Ltd.
Références
Arthritis Rheum. 2012 Oct;64(10):3083-94
pubmed: 22576262
Genome Biol. 2015 Apr 08;16:67
pubmed: 25887922
J Dent Res. 2018 Dec;97(13):1424-1430
pubmed: 30075090
Periodontol 2000. 2013 Feb;61(1):103-24
pubmed: 23240946
J Clin Periodontol. 2012 May;39(5):425-33
pubmed: 22417294
J Clin Periodontol. 2013 Mar;40(3):218-26
pubmed: 23294017
Clin Oral Implants Res. 2016 Jun;27(6):656-61
pubmed: 26354174
J Clin Periodontol. 2018 Dec;45(12):1498-1509
pubmed: 30341964
J Med Microbiol. 2018 Mar;67(3):332-340
pubmed: 29458668
J Clin Periodontol. 2021 Sep;48(9):1228-1239
pubmed: 34101220
Periodontol 2000. 2020 Oct;84(1):14-34
pubmed: 32844416
J Clin Periodontol. 2017 Dec;44(12):1274-1284
pubmed: 28766745
J Periodontol. 1998 Dec;69(12):1413-8
pubmed: 9926772
J Clin Periodontol. 1984 Sep;11(8):504-14
pubmed: 6384275
J Periodontol. 2018 Jun;89 Suppl 1:S267-S290
pubmed: 29926957
PLoS One. 2012;7(8):e42770
pubmed: 22900048
Int J Oral Maxillofac Implants. 2010 Jul-Aug;25(4):690-8
pubmed: 20657863
Clin Oral Implants Res. 1994 Dec;5(4):254-9
pubmed: 7640340
Clin Oral Implants Res. 2006 Feb;17(1):25-37
pubmed: 16441782
Nat Methods. 2010 Sep;7(9):668-9
pubmed: 20805793
J Oral Microbiol. 2010 May 24;2:
pubmed: 21523229
Periodontol 2000. 2020 Jun;83(1):26-39
pubmed: 32385877
Clin Oral Implants Res. 2019 Apr;30(4):306-314
pubmed: 30768875
Sci Rep. 2016 Aug 08;6:30997
pubmed: 27499042
J Dent. 2013 Dec;41(12):1297-301
pubmed: 24135296
Appl Environ Microbiol. 2007 Aug;73(16):5261-7
pubmed: 17586664
Virulence. 2015;6(3):183-7
pubmed: 25654499
J Periodontal Res. 2017 Dec;52(6):1021-1031
pubmed: 28707424
ISME J. 2013 May;7(5):1016-25
pubmed: 23303375
Bioinformatics. 2010 Oct 1;26(19):2460-1
pubmed: 20709691
J Periodontol. 2018 Jun;89 Suppl 1:S313-S318
pubmed: 29926955
Arch Oral Biol. 2017 Nov;83:145-152
pubmed: 28780383
J Dent Res. 2013 Dec;92(12 Suppl):168S-75S
pubmed: 24158341
Periodontol 2000. 2018 Feb;76(1):180-190
pubmed: 29239086
Genome Biol. 2010;11(8):R86
pubmed: 20738864
Clin Oral Implants Res. 2016 Mar;27(3):288-94
pubmed: 25529603
PeerJ. 2018 Apr 18;6:e4652
pubmed: 29682424
Clin Oral Implants Res. 2012 May;23(5):625-34
pubmed: 22093076
J Clin Periodontol. 2011 Mar;38 Suppl 11:203-13
pubmed: 21323716
J Periodontol. 2017 Oct;88(10):1066-1089
pubmed: 28625077
J Clin Med. 2020 Feb 01;9(2):
pubmed: 32024079
Periodontol 2000. 2005;38:135-87
pubmed: 15853940
J Periodontol. 2018 Jun;89 Suppl 1:S304-S312
pubmed: 29926953
J Periodontol. 1999 Feb;70(2):131-8
pubmed: 10102550
J Clin Periodontol. 1993 Nov;20(10):699-706
pubmed: 8276979
J Clin Periodontol. 2018 Apr;45(4):471-483
pubmed: 29331021
Clin Oral Implants Res. 2014 Oct;25(10):1192-9
pubmed: 23845046
J Clin Periodontol. 1995 Feb;22(2):124-30
pubmed: 7775668
Sci Rep. 2016 Feb 01;6:20205
pubmed: 26830979
Clin Implant Dent Relat Res. 2018 Dec;20(6):945-953
pubmed: 30255621
Genome Biol. 2011 Jun 24;12(6):R60
pubmed: 21702898
J Periodontal Res. 2018 Oct;53(5):657-681
pubmed: 29882313
J Clin Periodontol. 2002 Nov;29(11):1023-8
pubmed: 12472995
J Clin Periodontol. 2015 Dec;42(12):1074-82
pubmed: 26461079
Periodontol 2000. 2019 Oct;81(1):29-40
pubmed: 31407437
Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
pubmed: 9254694
Clin Implant Dent Relat Res. 2014 Jun;16(3):401-11
pubmed: 22967131
J Periodontal Res. 2019 Aug;54(4):405-412
pubmed: 30734922
Nat Methods. 2010 May;7(5):335-6
pubmed: 20383131
J Periodontal Res. 1989 Mar;24(2):96-105
pubmed: 2542514
Clin Oral Implants Res. 2007 Aug;18(4):501-8
pubmed: 17501978
Sci Rep. 2015 Jun 16;5:10948
pubmed: 26077225
FEMS Microbiol Rev. 2015 Jul;39(4):509-21
pubmed: 25701044
Database (Oxford). 2010 Jul 06;2010:baq013
pubmed: 20624719
BMC Biol. 2014 Nov 12;12:87
pubmed: 25387460
Bioinformatics. 2011 Aug 15;27(16):2194-200
pubmed: 21700674
Atherosclerosis. 2015 Dec;243(2):573-8
pubmed: 26536303