Journal

The Journal of neuroscience : the official journal of the Society for Neuroscience
ISSN: 1529-2401
Titre abrégé: J Neurosci
Pays: United States
ID NLM: 8102140

Informations de publication

Date de publication:
07 Jun 2021
Historique:
received: 16 12 2020
revised: 29 04 2021
accepted: 03 05 2021
entrez: 9 6 2021
pubmed: 10 6 2021
medline: 10 6 2021
Statut: aheadofprint

Résumé

Neuropeptide secretion from dense-core vesicles (DCVs) controls many brain functions. Several components of the DCV exocytosis machinery have recently been identified, but the participation of a SEC1/MUNC18 (SM) protein has remained elusive. Here, we tested the ability of the three exocytic SM proteins expressed in the mammalian brain, MUNC18-1/2/3, to support neuropeptide secretion. We quantified DCV exocytosis at a single vesicle resolution upon action potential train-stimulation in mouse CNS neurons (of unknown sex) using pHluorin- and/or mCherry-tagged Neuropeptide-Y (NPY) or Brain-Derived Neurotrophic Factor (BDNF). Conditional inactivation of

Identifiants

pubmed: 34103363
pii: JNEUROSCI.3150-20.2021
doi: 10.1523/JNEUROSCI.3150-20.2021
pmc: PMC8276746
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

Copyright © 2021 the authors.

Déclaration de conflit d'intérêts

The authors declare no competing financial interests.

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Auteurs

Daniël C Puntman (DC)

Section Functional genomics, Department of Clinical Genetics, Center for Neurogenomics and Cognitive Research (CNCR), UMC Amsterdam, The Netherlands.

Swati Arora (S)

Department of Functional Genomics, Center for Neurogenomics and Cognitive Research (CNCR), Vrije Universiteit (VU) Amsterdam, de Boelelaan 1087, 1081 HV Amsterdam, The Netherlands.

Margherita Farina (M)

Section Functional genomics, Department of Clinical Genetics, Center for Neurogenomics and Cognitive Research (CNCR), UMC Amsterdam, The Netherlands.

Ruud F Toonen (RF)

Department of Functional Genomics, Center for Neurogenomics and Cognitive Research (CNCR), Vrije Universiteit (VU) Amsterdam, de Boelelaan 1087, 1081 HV Amsterdam, The Netherlands matthijs@cncr.vu.nl ruud.toonen@cncr.vu.nl matthijs@cncr.vu.nl.

Matthijs Verhage (M)

Section Functional genomics, Department of Clinical Genetics, Center for Neurogenomics and Cognitive Research (CNCR), UMC Amsterdam, The Netherlands matthijs@cncr.vu.nl ruud.toonen@cncr.vu.nl matthijs@cncr.vu.nl.
Department of Functional Genomics, Center for Neurogenomics and Cognitive Research (CNCR), Vrije Universiteit (VU) Amsterdam, de Boelelaan 1087, 1081 HV Amsterdam, The Netherlands.

Classifications MeSH