A Microfluidic Multisize Spheroid Array for Multiparametric Screening of Anticancer Drugs and Blood-Brain Barrier Transport Properties.
Antineoplastic Agents
/ chemistry
Biological Transport
/ drug effects
Blood-Brain Barrier
/ drug effects
Doxorubicin
/ chemistry
Drug Evaluation, Preclinical
/ methods
Endothelial Cells
/ drug effects
Humans
Microfluidic Analytical Techniques
Neoplasms
/ drug therapy
Spheroids, Cellular
/ drug effects
anticancer drugs
blood-brain barrier
in vitro tests
microfluidics
multicellular spheroids
Journal
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
ISSN: 2198-3844
Titre abrégé: Adv Sci (Weinh)
Pays: Germany
ID NLM: 101664569
Informations de publication
Date de publication:
06 2021
06 2021
Historique:
revised:
30
01
2021
received:
16
12
2020
entrez:
9
6
2021
pubmed:
10
6
2021
medline:
15
12
2021
Statut:
ppublish
Résumé
Physiological-relevant in vitro tissue models with their promise of better predictability have the potential to improve drug screening outcomes in preclinical studies. Despite the advances of spheroid models in pharmaceutical screening applications, variations in spheroid size and consequential altered cell responses often lead to nonreproducible and unpredictable results. Here, a microfluidic multisize spheroid array is established and characterized using liver, lung, colon, and skin cells as well as a triple-culture model of the blood-brain barrier (BBB) to assess the effects of spheroid size on (a) anticancer drug toxicity and (b) compound penetration across an advanced BBB model. The reproducible on-chip generation of 360 spheroids of five dimensions on a well-plate format using an integrated microlens technology is demonstrated. While spheroid size-related IC
Identifiants
pubmed: 34105271
doi: 10.1002/advs.202004856
pmc: PMC8188192
doi:
Substances chimiques
Antineoplastic Agents
0
Doxorubicin
80168379AG
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2004856Subventions
Organisme : Christian Doppler Forschungsgesellschaft (Josef Ressel Center for Phytogenic Drug Research)
Organisme : European Union's Horizon 2020 research and innovation program
Organisme : (EFPIA) grant agreement number 807015
Informations de copyright
© 2021 The Authors. Advanced Science published by Wiley-VCH GmbH.
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