HP1α is a chromatin crosslinker that controls nuclear and mitotic chromosome mechanics.
HP1a
cell biology
chromosome
chromosomes
gene expression
heterochromatin
human
mechanics
mitosis
nucleus
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
09 06 2021
09 06 2021
Historique:
received:
12
10
2020
accepted:
08
06
2021
pubmed:
10
6
2021
medline:
30
11
2021
entrez:
9
6
2021
Statut:
epublish
Résumé
Chromatin, which consists of DNA and associated proteins, contains genetic information and is a mechanical component of the nucleus. Heterochromatic histone methylation controls nucleus and chromosome stiffness, but the contribution of heterochromatin protein HP1α (CBX5) is unknown. We used a novel HP1α auxin-inducible degron human cell line to rapidly degrade HP1α. Degradation did not alter transcription, local chromatin compaction, or histone methylation, but did decrease chromatin stiffness. Single-nucleus micromanipulation reveals that HP1α is essential to chromatin-based mechanics and maintains nuclear morphology, separate from histone methylation. Further experiments with dimerization-deficient HP1α
Identifiants
pubmed: 34106828
doi: 10.7554/eLife.63972
pii: 63972
pmc: PMC8233041
doi:
pii:
Substances chimiques
CBX5 protein, human
0
Chromatin
0
Chromosomal Proteins, Non-Histone
0
Chromobox Protein Homolog 5
107283-02-3
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NCI NIH HHS
ID : U01 CA200060
Pays : United States
Organisme : NIDDK NIH HHS
ID : U54 DK107980
Pays : United States
Organisme : NHGRI NIH HHS
ID : R01 HG009906
Pays : United States
Organisme : NIDA NIH HHS
ID : U01 DA040583
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM124820
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM105847
Pays : United States
Organisme : NIDA NIH HHS
ID : U01 DA040601
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States
Organisme : NIGMS NIH HHS
ID : R00 GM123195
Pays : United States
Organisme : NIDDK NIH HHS
ID : R24 DK106766
Pays : United States
Organisme : NCI NIH HHS
ID : U54 CA193419
Pays : United States
Informations de copyright
© 2021, Strom et al.
Déclaration de conflit d'intérêts
AS, RB, EB, XW, KC, CH, JC, FY, JR, LT, DS, AT, MG, CB, JM, AS No competing interests declared
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