Molecular ruler of the attachment organelle in Mycoplasma pneumoniae.
Journal
PLoS pathogens
ISSN: 1553-7374
Titre abrégé: PLoS Pathog
Pays: United States
ID NLM: 101238921
Informations de publication
Date de publication:
06 2021
06 2021
Historique:
received:
12
02
2021
accepted:
07
05
2021
entrez:
10
6
2021
pubmed:
11
6
2021
medline:
8
10
2021
Statut:
epublish
Résumé
Length control is a fundamental requirement for molecular architecture. Even small wall-less bacteria have specially developed macro-molecular structures to support their survival. Mycoplasma pneumoniae, a human pathogen, forms a polar extension called an attachment organelle, which mediates cell division, cytadherence, and cell movement at host cell surface. This characteristic ultrastructure has a constant size of 250-300 nm, but its design principle remains unclear. In this study, we constructed several mutants by genetic manipulation to increase or decrease coiled-coil regions of HMW2, a major component protein of 200 kDa aligned in parallel along the cell axis. HMW2-engineered mutants produced both long and short attachment organelles, which we quantified by transmission electron microscopy and fluorescent microscopy with nano-meter precision. This simple design of HMW2 acting as a molecular ruler for the attachment organelle should provide an insight into bacterial cellular organization and its function for their parasitic lifestyles.
Identifiants
pubmed: 34111235
doi: 10.1371/journal.ppat.1009621
pii: PPATHOGENS-D-21-00329
pmc: PMC8191905
doi:
Substances chimiques
Adhesins, Bacterial
0
HMW2 protein, bacteria
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1009621Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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