Analysis of multispectral imaging with the AstroPath platform informs efficacy of PD-1 blockade.

Adult Aged Aged, 80 and over Antigens, CD / analysis Antigens, Differentiation, Myelomonocytic / analysis Antineoplastic Agents, Immunological / therapeutic use B7-H1 Antigen / analysis Biomarkers, Tumor / analysis CD8 Antigens / analysis Female Fluorescent Antibody Technique Forkhead Transcription Factors / analysis Humans Immune Checkpoint Proteins / analysis Macrophages / chemistry Male Melanoma / chemistry Middle Aged Prognosis Programmed Cell Death 1 Receptor / analysis Progression-Free Survival Receptors, Cell Surface / analysis SOXE Transcription Factors / analysis Single-Cell Analysis T-Lymphocyte Subsets / chemistry Treatment Outcome Tumor Microenvironment

Journal

Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511

Informations de publication

Date de publication:
11 06 2021
Historique:
received: 26 11 2019
revised: 08 02 2021
accepted: 03 05 2021
entrez: 11 6 2021
pubmed: 12 6 2021
medline: 22 6 2021
Statut: ppublish

Résumé

Next-generation tissue-based biomarkers for immunotherapy will likely include the simultaneous analysis of multiple cell types and their spatial interactions, as well as distinct expression patterns of immunoregulatory molecules. Here, we introduce a comprehensive platform for multispectral imaging and mapping of multiple parameters in tumor tissue sections with high-fidelity single-cell resolution. Image analysis and data handling components were drawn from the field of astronomy. Using this "AstroPath" whole-slide platform and only six markers, we identified key features in pretreatment melanoma specimens that predicted response to anti-programmed cell death-1 (PD-1)-based therapy, including CD163

Identifiants

DOI: 10.1126/science.aba2609 PMID: 34112666 PMC: PMC8709533
pubmed: 34112666
pii: 372/6547/eaba2609
doi: 10.1126/science.aba2609
pmc: PMC8709533
mid: NIHMS1764053
pii:
doi:

Substances chimiques

Antigens, CD 0
Antigens, Differentiation, Myelomonocytic 0
Antineoplastic Agents, Immunological 0
B7-H1 Antigen 0
Biomarkers, Tumor 0
CD163 antigen 0
CD274 protein, human 0
CD8 Antigens 0
FOXP3 protein, human 0
Forkhead Transcription Factors 0
Immune Checkpoint Proteins 0
PDCD1 protein, human 0
Programmed Cell Death 1 Receptor 0
Receptors, Cell Surface 0
SOX10 protein, human 0
SOXE Transcription Factors 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NCI NIH HHS
ID : P50 CA121974
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA197296
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA142779
Pays : United States
Organisme : NCI NIH HHS
ID : P50 CA062924
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA006973
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR001863
Pays : United States
Organisme : NCI NIH HHS
ID : R50 CA243627
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA196390
Pays : United States
Organisme : NCI NIH HHS
ID : T32 CA193145
Pays : United States
Organisme : NCI NIH HHS
ID : P01 CA247886
Pays : United States

Commentaires et corrections

Type : CommentIn

Informations de copyright

Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.

Références

Sci Rep. 2017 Oct 17;7(1):13380
pubmed: 29042640
J Histochem Cytochem. 2007 Feb;55(2):105-9
pubmed: 16982846
Sci Immunol. 2016 Jul 14;1(1):aaf6925
pubmed: 28783673
JCI Insight. 2017 Jul 20;2(14):
pubmed: 28724788
J Immunother Cancer. 2018 Oct 1;6(1):99
pubmed: 30285852
Science. 2012 Nov 30;338(6111):1220-5
pubmed: 23197535
J Immunother Cancer. 2018 Jun 6;6(1):48
pubmed: 29871672
Mod Pathol. 2018 Feb;31(2):214-234
pubmed: 29192647
Science. 2015 Apr 3;348(6230):124-8
pubmed: 25765070
Proc Natl Acad Sci U S A. 2008 Sep 30;105(39):15016-21
pubmed: 18809920
Science. 2020 Jan 31;367(6477):
pubmed: 32001626
Nat Immunol. 2019 Nov;20(11):1425-1434
pubmed: 31611702
Sci Transl Med. 2012 Mar 28;4(127):127ra37
pubmed: 22461641
Nat Med. 2019 Mar;25(3):470-476
pubmed: 30742120
Cancer Immunol Res. 2018 Apr;6(4):481-493
pubmed: 29467127
Curr Opin Immunol. 2017 Apr;45:73-81
pubmed: 28236750
Clin Cancer Res. 2019 Jun 1;25(11):3455-3467
pubmed: 30808776
Nat Rev Cancer. 2019 Apr;19(4):215-227
pubmed: 30867580
J Immunother Cancer. 2019 Feb 12;7(1):43
pubmed: 30755279
J Immunol. 2018 Jan 1;200(1):347-354
pubmed: 29141863
Immunity. 2019 Jan 15;50(1):181-194.e6
pubmed: 30635236
Cancer Res. 2017 Feb 15;77(4):851-861
pubmed: 27979840
Nat Immunol. 2020 Oct;21(10):1232-1243
pubmed: 32929275
Science. 2001 Sep 14;293(5537):2037-40
pubmed: 11557879
Nat Med. 2018 Jul;24(7):994-1004
pubmed: 29892065
Nat Rev Cancer. 2012 Mar 15;12(4):298-306
pubmed: 22419253
Nat Rev Immunol. 2015 Aug;15(8):486-99
pubmed: 26205583
Sci Transl Med. 2018 Jul 18;10(450):
pubmed: 30021886
Arch Pathol Lab Med. 2014 Nov;138(11):1432-43
pubmed: 24646069
Cell. 2018 Sep 6;174(6):1373-1387.e19
pubmed: 30193111
Nat Rev Cancer. 2012 Mar 22;12(4):252-64
pubmed: 22437870
Nat Med. 2019 Apr;25(4):656-666
pubmed: 30833750
Nature. 2017 Jan 18;541(7637):321-330
pubmed: 28102259
Int J Cancer. 2011 Aug 1;129(3):636-47
pubmed: 20857491
Curr Opin Pharmacol. 2015 Aug;23:32-8
pubmed: 26047524
Cancer Inform. 2013 Sep 23;12:193-201
pubmed: 24092958
Cell Rep. 2017 Apr 4;19(1):203-217
pubmed: 28380359
Nature. 2006 Mar 23;440(7083):413-4
pubmed: 16554783
JAMA Oncol. 2019 Aug 1;5(8):1195-1204
pubmed: 31318407
Science. 2017 Jul 28;357(6349):409-413
pubmed: 28596308
J Immunother Cancer. 2020 May;8(1):
pubmed: 32414858
Cell. 2019 Oct 31;179(4):846-863.e24
pubmed: 31668803
Clin Cancer Res. 2017 Aug 15;23(16):4938-4944
pubmed: 28428193
Cancer Res. 2010 Nov 1;70(21):8378-87
pubmed: 20861189

Auteurs

Sneha Berry (S)

The Mark Foundation Center for Advanced Genomics and Imaging, Johns Hopkins University, Baltimore, MD 21287, USA.
Bloomberg~Kimmel Institute for Cancer Immunotherapy and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD 21287, USA.
Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
ORCID: 0000-0002-4887-8969

Nicolas A Giraldo (NA)

Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
ORCID: 0000-0002-7452-1103

Benjamin F Green (BF)

The Mark Foundation Center for Advanced Genomics and Imaging, Johns Hopkins University, Baltimore, MD 21287, USA.
Bloomberg~Kimmel Institute for Cancer Immunotherapy and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD 21287, USA.
Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.

Tricia R Cottrell (TR)

Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
ORCID: 0000-0003-2952-1830

Julie E Stein (JE)

Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
ORCID: 0000-0002-8040-5205

Elizabeth L Engle (EL)

The Mark Foundation Center for Advanced Genomics and Imaging, Johns Hopkins University, Baltimore, MD 21287, USA.
Bloomberg~Kimmel Institute for Cancer Immunotherapy and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD 21287, USA.
Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.

Haiying Xu (H)

The Mark Foundation Center for Advanced Genomics and Imaging, Johns Hopkins University, Baltimore, MD 21287, USA.
Bloomberg~Kimmel Institute for Cancer Immunotherapy and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD 21287, USA.
Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.

Aleksandra Ogurtsova (A)

The Mark Foundation Center for Advanced Genomics and Imaging, Johns Hopkins University, Baltimore, MD 21287, USA.
Bloomberg~Kimmel Institute for Cancer Immunotherapy and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD 21287, USA.
Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.

Charles Roberts (C)

Bloomberg~Kimmel Institute for Cancer Immunotherapy and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD 21287, USA.
Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.

Daphne Wang (D)

Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
ORCID: 0000-0002-7202-8075

Peter Nguyen (P)

Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.

Qingfeng Zhu (Q)

Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
ORCID: 0000-0001-7871-3359

Sigfredo Soto-Diaz (S)

Bloomberg~Kimmel Institute for Cancer Immunotherapy and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD 21287, USA.
Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.

Jose Loyola (J)

Bloomberg~Kimmel Institute for Cancer Immunotherapy and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD 21287, USA.
Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
ORCID: 0000-0002-0746-2174

Inbal B Sander (IB)

Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.

Pok Fai Wong (PF)

Department of Pathology, Yale University School of Medicine, New Haven, CT 06510, USA.

Shlomit Jessel (S)

Department of Pathology, Yale University School of Medicine, New Haven, CT 06510, USA.
ORCID: 0000-0001-8489-0351

Joshua Doyle (J)

Department of Astronomy and Physics, Johns Hopkins University, Baltimore, MD 21218, USA.
Institute for Data Intensive Engineering and Science, Johns Hopkins University, Baltimore, MD 21218, USA.

Danielle Signer (D)

Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
ORCID: 0000-0002-5825-7525

Richard Wilton (R)

Department of Astronomy and Physics, Johns Hopkins University, Baltimore, MD 21218, USA.
Institute for Data Intensive Engineering and Science, Johns Hopkins University, Baltimore, MD 21218, USA.
ORCID: 0000-0003-1263-5532

Jeffrey S Roskes (JS)

Department of Astronomy and Physics, Johns Hopkins University, Baltimore, MD 21218, USA.
Institute for Data Intensive Engineering and Science, Johns Hopkins University, Baltimore, MD 21218, USA.
ORCID: 0000-0001-8761-0490

Margaret Eminizer (M)

Department of Astronomy and Physics, Johns Hopkins University, Baltimore, MD 21218, USA.
Institute for Data Intensive Engineering and Science, Johns Hopkins University, Baltimore, MD 21218, USA.
ORCID: 0000-0003-4591-2225

Seyoun Park (S)

The Mark Foundation Center for Advanced Genomics and Imaging, Johns Hopkins University, Baltimore, MD 21287, USA.
Department of Radiology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
ORCID: 0000-0002-6987-9362

Joel C Sunshine (JC)

Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
ORCID: 0000-0001-9987-6712

Elizabeth M Jaffee (EM)

The Mark Foundation Center for Advanced Genomics and Imaging, Johns Hopkins University, Baltimore, MD 21287, USA.
Bloomberg~Kimmel Institute for Cancer Immunotherapy and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD 21287, USA.
Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
ORCID: 0000-0003-3841-6549

Alexander Baras (A)

Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
ORCID: 0000-0003-2397-3342

Angelo M De Marzo (AM)

Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
ORCID: 0000-0003-4847-5307

Suzanne L Topalian (SL)

Bloomberg~Kimmel Institute for Cancer Immunotherapy and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD 21287, USA.
Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
ORCID: 0000-0002-0821-8587

Harriet Kluger (H)

Division of Medical Oncology, Department of Medicine, Yale University School of Medicine, New Haven, CT 06510, USA.

Leslie Cope (L)

The Mark Foundation Center for Advanced Genomics and Imaging, Johns Hopkins University, Baltimore, MD 21287, USA.
Bloomberg~Kimmel Institute for Cancer Immunotherapy and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD 21287, USA.
Division of Biostatistics and Bioinformatics, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
ORCID: 0000-0002-4098-3289

Evan J Lipson (EJ)

The Mark Foundation Center for Advanced Genomics and Imaging, Johns Hopkins University, Baltimore, MD 21287, USA.
Bloomberg~Kimmel Institute for Cancer Immunotherapy and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD 21287, USA.
Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
ORCID: 0000-0003-2976-0911

Ludmila Danilova (L)

The Mark Foundation Center for Advanced Genomics and Imaging, Johns Hopkins University, Baltimore, MD 21287, USA.
Bloomberg~Kimmel Institute for Cancer Immunotherapy and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD 21287, USA.
Division of Biostatistics and Bioinformatics, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
ORCID: 0000-0003-2813-3094

Robert A Anders (RA)

The Mark Foundation Center for Advanced Genomics and Imaging, Johns Hopkins University, Baltimore, MD 21287, USA.
Bloomberg~Kimmel Institute for Cancer Immunotherapy and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD 21287, USA.
Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.

David L Rimm (DL)

Department of Pathology, Yale University School of Medicine, New Haven, CT 06510, USA.
ORCID: 0000-0001-5820-4397

Drew M Pardoll (DM)

The Mark Foundation Center for Advanced Genomics and Imaging, Johns Hopkins University, Baltimore, MD 21287, USA.
Bloomberg~Kimmel Institute for Cancer Immunotherapy and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD 21287, USA.
Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
ORCID: 0000-0001-6215-1013

Alexander S Szalay (AS)

The Mark Foundation Center for Advanced Genomics and Imaging, Johns Hopkins University, Baltimore, MD 21287, USA.
Department of Astronomy and Physics, Johns Hopkins University, Baltimore, MD 21218, USA.
Institute for Data Intensive Engineering and Science, Johns Hopkins University, Baltimore, MD 21218, USA.
ORCID: 0000-0002-4108-3282

Janis M Taube (JM)

The Mark Foundation Center for Advanced Genomics and Imaging, Johns Hopkins University, Baltimore, MD 21287, USA. jtaube1@jhmi.edu.
Bloomberg~Kimmel Institute for Cancer Immunotherapy and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD 21287, USA.
Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
ORCID: 0000-0002-8273-4395

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female

Evaluation of Low-Value Services Across Major Medicare Advantage Insurers and Traditional Medicare.

Ciara Duggan, Adam L Beckman, Ishani Ganguli et al.
1.00
Humans United States Aged Cross-Sectional Studies Medicare Part C

Effectiveness of Virtual Yoga for Chronic Low Back Pain: A Randomized Clinical Trial.

Hallie Tankha, Devyn Gaskins, Amanda Shallcross et al.
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH

questionsmedicales.fr › Personnes › Tranches d'âge › Adulte › Analysis of multispectral imaging with the...
questionsmedicales.fr › Personnes › Tranches d'âge › Adulte › Sujet âgé › Analysis of multispectral imaging with the...
questionsmedicales.fr › Personnes › Tranches d'âge › Adulte › Sujet âgé › Sujet âgé de 80 ans ou plus › Analysis of multispectral imaging with the...
questionsmedicales.fr › Facteurs biologiques › Marqueurs biologiques › Antigènes de différenciation › Antigènes CD › Analysis of multispectral imaging with the...
questionsmedicales.fr › Facteurs biologiques › Marqueurs biologiques › Antigènes de différenciation › Antigènes de différenciation des myélomonocytes › Analysis of multispectral imaging with the...
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Antinéoplasiques › Antinéoplasiques immunologiques › Analysis of multispectral imaging with the...
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Antigènes B7 › Antigène CD274 › Analysis of multispectral imaging with the...
questionsmedicales.fr › Facteurs biologiques › Marqueurs biologiques › Marqueurs biologiques tumoraux › Analysis of multispectral imaging with the...
questionsmedicales.fr › Facteurs biologiques › Marqueurs biologiques › Antigènes de différenciation › Antigènes de différenciation des lymphocytes T › Antigènes CD8 › Analysis of multispectral imaging with the...
questionsmedicales.fr › Techniques d'investigation › Techniques immunologiques › Technique d'immunofluorescence › Analysis of multispectral imaging with the...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Facteurs de transcription à motif hélice ailée › Facteurs de transcription Forkhead › Analysis of multispectral imaging with the...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Analysis of multispectral imaging with the...
questionsmedicales.fr › Facteurs biologiques › Protéines de points de contrôle immunitaires › Analysis of multispectral imaging with the...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Phagocytes › Macrophages › Analysis of multispectral imaging with the...
questionsmedicales.fr › Tumeurs › Tumeurs par type histologique › Naevus et mélanomes › Mélanome › Analysis of multispectral imaging with the...
questionsmedicales.fr › Personnes › Tranches d'âge › Adulte › Adulte d'âge moyen › Analysis of multispectral imaging with the...
questionsmedicales.fr › Diagnostic › Pronostic › Analysis of multispectral imaging with the...
questionsmedicales.fr › Facteurs biologiques › Marqueurs biologiques › Antigènes de différenciation › Antigènes de différenciation des lymphocytes T › Récepteur-1 de mort cellulaire programmée › Analysis of multispectral imaging with the...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Statistiques comme sujet › Analyse de survie › Survie sans progression › Analysis of multispectral imaging with the...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Analysis of multispectral imaging with the...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Facteurs de transcription SOX › Facteurs de transcription SOX-E › Analysis of multispectral imaging with the...
questionsmedicales.fr › Techniques d'investigation › Techniques cytologiques › Analyse sur cellule unique › Analysis of multispectral imaging with the...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Agranulocytes › Lymphocytes › Lymphocytes T › Sous-populations de lymphocytes T › Analysis of multispectral imaging with the...
questionsmedicales.fr › Qualité, accès, évaluation des soins de santé › Qualité des soins de santé › Mécanismes d'évaluation des soins de santé › Évaluation des résultats et des processus en soins de santé › Évaluation de résultat (soins) › Résultat thérapeutique › Analysis of multispectral imaging with the...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Microenvironnement cellulaire › Microenvironnement tumoral › Analysis of multispectral imaging with the...