Single-molecule amplification-free multiplexed detection of circulating microRNA cancer biomarkers from serum.
Biomarkers, Tumor
/ analysis
Circulating MicroRNA
/ analysis
Early Detection of Cancer
/ instrumentation
Fluorescence
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
/ genetics
Humans
Liquid Biopsy
Male
MicroRNAs
/ analysis
Nanopores
Prostatic Neoplasms
/ blood
Real-Time Polymerase Chain Reaction
Sensitivity and Specificity
Single Molecule Imaging
/ methods
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
10 06 2021
10 06 2021
Historique:
received:
13
01
2021
accepted:
30
04
2021
entrez:
11
6
2021
pubmed:
12
6
2021
medline:
30
6
2021
Statut:
epublish
Résumé
MicroRNAs (miRNAs) play essential roles in post-transcriptional gene expression and are also found freely circulating in bodily fluids such as blood. Dysregulated miRNA signatures have been associated with many diseases including cancer, and miRNA profiling from liquid biopsies offers a promising strategy for cancer diagnosis, prognosis and monitoring. Here, we develop size-encoded molecular probes that can be used for simultaneous electro-optical nanopore sensing of miRNAs, allowing for ultrasensitive, sequence-specific and multiplexed detection directly in unprocessed human serum, in sample volumes as small as 0.1 μl. We show that this approach allows for femtomolar sensitivity and single-base mismatch selectivity. We demonstrate the ability to simultaneously monitor miRNAs (miR-141-3p and miR-375-3p) from prostate cancer patients with active disease and in remission. This technology can pave the way for next generation of minimally invasive diagnostic and companion diagnostic tests for cancer.
Identifiants
pubmed: 34112774
doi: 10.1038/s41467-021-23497-y
pii: 10.1038/s41467-021-23497-y
pmc: PMC8192752
doi:
Substances chimiques
Biomarkers, Tumor
0
Circulating MicroRNA
0
MIRN141 microRNA, human
0
MIRN375 microRNA, human
0
MicroRNAs
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3515Subventions
Organisme : Cancer Research UK
ID : C49996/A26141
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/R022429/1
Pays : United Kingdom
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