In situ strategy for biomedical target localization via nanogold nucleation and secondary growth.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
10 06 2021
10 06 2021
Historique:
received:
23
11
2020
accepted:
19
05
2021
entrez:
11
6
2021
pubmed:
12
6
2021
medline:
11
8
2021
Statut:
epublish
Résumé
Immunocytochemistry visualizes the exact spatial location of target molecules. The most common strategy for ultrastructural immunocytochemistry is the conjugation of nanogold particles to antibodies as probes. However, conventional nanogold labelling requires time-consuming nanogold probe preparation and ultrathin sectioning of cell/tissue samples. Here, we introduce an in situ strategy involving nanogold nucleation in immunoenzymatic products on universal paraffin/cryostat sections and provide unique insight into nanogold development under hot-humid air conditions. Nanogold particles were specifically localized on kidney podocytes to target synaptopodin. Transmission electron microscopy revealed secondary growth and self-assembly that could be experimentally controlled by bovine serum albumin stabilization and phosphate-buffered saline acceleration. Valuable retrospective nanogold labelling for gastric H
Identifiants
pubmed: 34112923
doi: 10.1038/s42003-021-02246-3
pii: 10.1038/s42003-021-02246-3
pmc: PMC8192519
doi:
Substances chimiques
Microfilament Proteins
0
Synpo protein, rat
0
Gold
7440-57-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
710Références
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