Phagocytosis and Efferocytosis by Resident Macrophages in the Mouse Pancreas.
efferocytosis
in vivo imaging
macrophages
pancreas
phagocytosis
type 1 diabetes
Journal
Frontiers in endocrinology
ISSN: 1664-2392
Titre abrégé: Front Endocrinol (Lausanne)
Pays: Switzerland
ID NLM: 101555782
Informations de publication
Date de publication:
2021
2021
Historique:
received:
14
09
2020
accepted:
28
04
2021
entrez:
11
6
2021
pubmed:
12
6
2021
medline:
11
1
2022
Statut:
epublish
Résumé
The tissue microenvironment in the mouse pancreas has been shown to promote very different polarizations of resident macrophages with islet-resident macrophages displaying an inflammatory "M1" profile and macrophages in the exocrine tissue mostly displaying an alternatively activated "M2" profile. The impact of this polarization on tissue homeostasis and diabetes development is unclear. In this study, the ability of pancreas-resident macrophages to phagocyte bacterial and endogenous debris was investigated. Mouse endocrine and exocrine tissues were separated, and tissue-resident macrophages were isolated by magnetic immunolabeling. Isolated macrophages were subjected to flow cytometry for polarization markers and qPCR for phagocytosis-related genes. Functional
Identifiants
pubmed: 34113315
doi: 10.3389/fendo.2021.606175
pmc: PMC8185276
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
606175Informations de copyright
Copyright © 2021 Parv, Westerlund, Merchant, Komijani, Lindsay and Christoffersson.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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