Full assembly of HIV-1 particles requires assistance of the membrane curvature factor IRSp53.
physics of living systems
plasma membrane
single molecule localisation microscopy
virus
viruses
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
11 06 2021
11 06 2021
Historique:
received:
07
02
2021
accepted:
10
06
2021
pubmed:
12
6
2021
medline:
21
10
2021
entrez:
11
6
2021
Statut:
epublish
Résumé
During HIV-1 particle formation, the requisite plasma membrane curvature is thought to be solely driven by the retroviral Gag protein. Here, we reveal that the cellular I-BAR protein IRSp53 is required for the progression of HIV-1 membrane curvature to complete particle assembly. siRNA-mediated knockdown of IRSp53 gene expression induces a decrease in viral particle production and a viral bud arrest at half completion. Single-molecule localization microscopy at the cell plasma membrane shows a preferential localization of IRSp53 around HIV-1 Gag assembly sites. In addition, we observe the presence of IRSp53 in purified HIV-1 particles. Finally, HIV-1 Gag protein preferentially localizes to curved membranes induced by IRSp53 I-BAR domain on giant unilamellar vesicles. Overall, our data reveal a strong interplay between IRSp53 I-BAR and Gag at membranes during virus assembly. This highlights IRSp53 as a crucial host factor in HIV-1 membrane curvature and its requirement for full HIV-1 particle assembly.
Identifiants
pubmed: 34114563
doi: 10.7554/eLife.67321
pii: 67321
pmc: PMC8260224
doi:
pii:
Substances chimiques
BAIAP2 protein, human
0
Nerve Tissue Proteins
0
gag Gene Products, Human Immunodeficiency Virus
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2021, Inamdar et al.
Déclaration de conflit d'intérêts
KI, FT, RD, Ad, JM, PM, RM, PR, JM, CF, DM No competing interests declared, PL, PB Reviewing editor, eLife
Références
Nat Methods. 2015 Nov;12(11):1065-71
pubmed: 26344046
Biophys J. 2013 Jul 2;105(1):154-64
pubmed: 23823234
Histochem Cell Biol. 2012 Jan;137(1):1-10
pubmed: 22086768
Cell. 2003 Sep 19;114(6):701-13
pubmed: 14505570
J Virol. 2015 Aug;89(16):8162-81
pubmed: 26018170
J Biol Chem. 1996 Feb 9;271(6):2921-8
pubmed: 8621681
J Microsc. 2006 Dec;224(Pt 3):213-32
pubmed: 17210054
Biophys J. 2021 Jan 5;120(1):46-54
pubmed: 33248130
Nat Struct Mol Biol. 2014 Apr;21(4):413-22
pubmed: 24584464
Proc Natl Acad Sci U S A. 2014 Aug 19;111(33):12211-6
pubmed: 25099357
Biophys J. 1993 Apr;64(4):1133-8
pubmed: 19431884
Nat Commun. 2018 May 9;9(1):1835
pubmed: 29743604
Cell. 2001 Oct 5;107(1):55-65
pubmed: 11595185
Proc Natl Acad Sci U S A. 2017 Nov 21;114(47):E10056-E10065
pubmed: 29114055
Biophys J. 2019 Aug 6;117(3):553-562
pubmed: 31349990
FEBS Lett. 2007 May 22;581(11):2089-97
pubmed: 17434167
Trends Cell Biol. 2008 Feb;18(2):52-60
pubmed: 18215522
Nat Commun. 2019 Jan 29;10(1):483
pubmed: 30696821
Biochem Biophys Res Commun. 2002 Apr 26;293(1):93-9
pubmed: 12054568
FEBS Lett. 2014 Aug 19;588(16):2693-703
pubmed: 24548561
J Cell Sci. 2007 May 1;120(Pt 9):1663-72
pubmed: 17430976
J Virol. 2003 Jun;77(11):6507-19
pubmed: 12743307
Nat Commun. 2015 Oct 15;6:8529
pubmed: 26469246
EMBO J. 2013 Oct 16;32(20):2735-50
pubmed: 24076653
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Sci Rep. 2016 Dec 23;6:39332
pubmed: 28008947
Sci Adv. 2019 Oct 02;5(10):eaaw8651
pubmed: 31616784
Cell. 1989 Oct 6;59(1):103-12
pubmed: 2676191
Cell. 2010 Dec 10;143(6):875-87
pubmed: 21145455
Nat Rev Mol Cell Biol. 2006 Jul;7(7):495-504
pubmed: 16773132
Curr Biol. 2001 Oct 30;11(21):1645-55
pubmed: 11696321
Elife. 2018 Jul 04;7:
pubmed: 29972351
Bioinformatics. 2014 Aug 15;30(16):2389-90
pubmed: 24771516
Curr Biol. 2009 Jan 27;19(2):95-107
pubmed: 19150238
Biochim Biophys Acta Gen Subj. 2018 Jun;1862(6):1421-1431
pubmed: 29571744
Elife. 2015 Jan 29;4:
pubmed: 25633977
Cell Rep. 2013 Sep 26;4(6):1213-23
pubmed: 24055060
Methods Enzymol. 2009;465:161-76
pubmed: 19913167
Sci Rep. 2017 Jan 09;7:40485
pubmed: 28067313
J Cell Biol. 2003 Aug 4;162(3):425-34
pubmed: 12900394
Nat Methods. 2008 Feb;5(2):155-7
pubmed: 18193054
Nat Cell Biol. 2019 Apr;21(4):452-461
pubmed: 30936472
Nature. 2000 Dec 7;408(6813):732-5
pubmed: 11130076
Retrovirology. 2009 Mar 11;6:28
pubmed: 19284574
Proc Natl Acad Sci U S A. 2014 Dec 2;111(48):E5205-13
pubmed: 25404326
Proc Natl Acad Sci U S A. 2004 Oct 12;101(41):14889-94
pubmed: 15465916
J Cell Biol. 2007 Mar 26;176(7):953-64
pubmed: 17371834
J Mol Biol. 2006 Jun 16;359(4):848-62
pubmed: 16682056
Proc Natl Acad Sci U S A. 2014 Jul 1;111(26):E2676-83
pubmed: 24938790
J Biol Chem. 2006 Nov 17;281(46):35347-58
pubmed: 17003044
Eur Phys J E Soft Matter. 2014 May;37(5):42
pubmed: 24859281
Sci Rep. 2017 Jul 28;7(1):6808
pubmed: 28754893
J Virol. 2004 Jun;78(11):5686-97
pubmed: 15140966
J Cell Biol. 2011 Dec 26;195(7):1071-82
pubmed: 22123832
Biophys J. 2013 Nov 19;105(10):2262-72
pubmed: 24268138
Sci Rep. 2018 Nov 2;8(1):16283
pubmed: 30389967
Curr Opin Cell Biol. 2011 Feb;23(1):14-21
pubmed: 21093245