Identification of Antibody Biomarker Using High-Density Nucleic Acid Programmable Protein Array.
Antibody biomarker discovery
Biomarker
HD-NAPPA
Nucleic acid programmable protein array
Silicon nanowells
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
11
6
2021
pubmed:
12
6
2021
medline:
26
8
2021
Statut:
ppublish
Résumé
A novel protein microarray technology, called high-density nucleic acid programmable protein array (HD-NAPPA), enables the serological screening of thousands of proteins at one time. HD-NAPPA extends the capabilities of NAPPA, which produces protein microarrays on a conventional glass microscope slide. By comparison, HD-NAPPA displays proteins in over 10,000 nanowells etched in a silicon slide. Proteins on HD-NAPPA are expressed in the individual isolated nanowells, via in vitro transcription and translation (IVTT), without any diffusion during incubation. Here we describe the method for antibody biomarker identification using HD-NAPPA, including four main steps: (1) HD-NAPPA array protein expression, (2) primary antibodies (serum/plasma) probing, (3) secondary antibody visualization, and (4) image scanning and data processing.
Identifiants
pubmed: 34115351
doi: 10.1007/978-1-0716-1562-1_4
doi:
Substances chimiques
Antibodies
0
Biomarkers
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
47-64Références
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