Enhanced isomerization of rare sugars by ribose-5-phosphate isomerase A from Ochrobactrum sp. CSL1.

Aldose-Ketose Isomerases / genetics Isomerism Ochrobactrum / metabolism Sugars
Alanine scanning D-allose Enzyme engineering Molecular dynamics Ochrobactrum sp. CSL1 ribose-5-phosphate isomerase A

Journal

Enzyme and microbial technology
ISSN: 1879-0909
Titre abrégé: Enzyme Microb Technol
Pays: United States
ID NLM: 8003761

Informations de publication

Date de publication:
Aug 2021
Historique:
received: 30 10 2020
revised: 19 03 2021
accepted: 22 03 2021
entrez: 12 6 2021
pubmed: 13 6 2021
medline: 19 8 2021
Statut: ppublish

Résumé

Ribose-5-phosphate isomerase A (RpiA) is of great importance in biochemistry research, however its application in biotechnology has not been fully explored. In this study the activity of RpiA from Ochrobactrum sp. CSL1 (OsRpiA) towards D-allose was engineered based on sequential and structural analyses. Strategies of alanine scanning, rational design and saturated mutagenesis were employed to create three mutant libraries. A single mutant of K124A showed a 45 % activity improvement towards D-allose. The reaction properties of the mutant were analyzed, and a shift of optimal pH and higher thermal stability at low reaction temperatures were identified. The conversion of D-allose was also improved by 40 % using K124A, and higher activities on major substrates were found in the mutant's substrate scope, implying its application potential in rare sugar preparation. Kinetics analysis revealed that K

Identifiants

DOI: 10.1016/j.enzmictec.2021.109789 PMID: 34116752
pubmed: 34116752
pii: S0141-0229(21)00047-8
doi: 10.1016/j.enzmictec.2021.109789
pii:
doi:

Substances chimiques

Sugars 0
Aldose-Ketose Isomerases EC 5.3.1.-
ribosephosphate isomerase EC 5.3.1.6

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

109789

Informations de copyright

Copyright © 2021 Elsevier Inc. All rights reserved.

Auteurs

Rong Wang (R)

School of Chemistry and Life Sciences, Suzhou University of Science and Technology, Suzhou, 215009, PR China.

Xinqi Xu (X)

Fujian Key Laboratory of Marine Enzyme Engineering, College of Biosciences and Engineering, Fuzhou University, Fuzhou, 350116, PR China.

Xuemei Yao (X)

School of Chemistry and Life Sciences, Suzhou University of Science and Technology, Suzhou, 215009, PR China.

Hengtao Tang (H)

School of Chemistry and Life Sciences, Suzhou University of Science and Technology, Suzhou, 215009, PR China.

Xin Ju (X)

School of Chemistry and Life Sciences, Suzhou University of Science and Technology, Suzhou, 215009, PR China. Electronic address: juxin@mail.usts.edu.cn.

Liangzhi Li (L)

School of Chemistry and Life Sciences, Suzhou University of Science and Technology, Suzhou, 215009, PR China. Electronic address: liliangzhi01@163.com.

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Classifications MeSH

questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Isomerases › Intramolecular oxidoreductases › Aldose-ketose isomerases › Enhanced isomerization of rare sugars by...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes de chimie organique › Isomérie › Enhanced isomerization of rare sugars by...
questionsmedicales.fr › Bactéries › Proteobacteria › Alphaproteobacteria › Brucellaceae › Ochrobactrum › Enhanced isomerization of rare sugars by...
questionsmedicales.fr › Glucides › Sucres › Enhanced isomerization of rare sugars by...