Features of hippocampal astrocytic domains and their spatial relation to excitatory and inhibitory neurons.
CLARITY
VIP
amygdala
hippocampus
parvalbumin
pyramidal neurons
somatostatin neurons
Journal
Glia
ISSN: 1098-1136
Titre abrégé: Glia
Pays: United States
ID NLM: 8806785
Informations de publication
Date de publication:
10 2021
10 2021
Historique:
revised:
28
05
2021
received:
10
02
2021
accepted:
01
06
2021
pubmed:
13
6
2021
medline:
12
3
2022
entrez:
12
6
2021
Statut:
ppublish
Résumé
The mounting evidence for the involvement of astrocytes in neuronal circuits function and behavior stands in stark contrast to the lack of detailed anatomical description of these cells and the neurons in their domains. To fill this void, we imaged >30,000 astrocytes in hippocampi made transparent by CLARITY, and determined the elaborate structure, distribution, and neuronal content of astrocytic domains. First, we characterized the spatial distribution of >19,000 astrocytes across CA1 lamina, and analyzed the morphology of thousands of reconstructed domains. We then determined the excitatory somatic content of CA1 astrocytes, and measured the distance between inhibitory neuronal somata to the nearest astrocyte soma. We find that on average, there are almost 14 pyramidal neurons per domain in the CA1, increasing toward the pyramidal layer midline, compared to only five excitatory neurons per domain in the amygdala. Finally, we discovered that somatostatin neurons are found in close proximity to astrocytes, compared to parvalbumin and VIP inhibitory neurons. This work provides a comprehensive large-scale quantitative foundation for studying neuron-astrocyte interactions.
Identifiants
pubmed: 34117643
doi: 10.1002/glia.24044
pmc: PMC7612014
mid: EMS129240
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2378-2390Subventions
Organisme : European Research Council
ID : 803589
Pays : International
Informations de copyright
© 2021 Wiley Periodicals LLC.
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