Biochemistry, structure, and cellular internalization of a four nanobody-bearing Fc dimer.
Amino Acid Sequence
Animals
Antigen-Antibody Complex
/ chemistry
Antigens
/ chemistry
Camelus
Cell Line, Tumor
Cloning, Molecular
Escherichia coli
/ genetics
Gene Expression
Genetic Vectors
/ chemistry
Humans
Immunoglobulin Fc Fragments
/ chemistry
Molecular Weight
Protein Binding
Protein Engineering
/ methods
Protein Multimerization
Receptor, ErbB-2
/ chemistry
Recombinant Fusion Proteins
/ chemistry
Recombinant Proteins
/ chemistry
Single-Domain Antibodies
/ chemistry
Trastuzumab
/ chemistry
VHH
antibody like molecules
cellular uptake
characterization
subcellular localization
Journal
Protein science : a publication of the Protein Society
ISSN: 1469-896X
Titre abrégé: Protein Sci
Pays: United States
ID NLM: 9211750
Informations de publication
Date de publication:
09 2021
09 2021
Historique:
revised:
11
06
2021
received:
27
02
2021
accepted:
11
06
2021
pubmed:
13
6
2021
medline:
4
2
2022
entrez:
12
6
2021
Statut:
ppublish
Résumé
VHH stands for the variable regions of heavy chain only of camelid IgGs. The VHH family forms a set of interesting proteins derived from antibodies that maintain their capacity to recognize the antigen, despite their relatively small molecular weight (in the 12,000 Da range). Continuing our exploration of the possibilities of those molecules, we chose to design alternative molecules with maintained antigen recognition, but enhanced capacity, by fusing four VHH with one Fc, the fragment crystallizable region of antibodies. In doing so, we aimed at having a molecule with superior quantitative antigen recognition (×4) while maintaining its size below the 110 kDa. In the present paper, we described the building of those molecules that we coined VHH
Identifiants
pubmed: 34117809
doi: 10.1002/pro.4147
pmc: PMC8376420
doi:
Substances chimiques
Antigen-Antibody Complex
0
Antigens
0
Immunoglobulin Fc Fragments
0
Recombinant Fusion Proteins
0
Recombinant Proteins
0
Single-Domain Antibodies
0
ERBB2 protein, human
EC 2.7.10.1
Receptor, ErbB-2
EC 2.7.10.1
Trastuzumab
P188ANX8CK
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1946-1957Informations de copyright
© 2021 The Protein Society.
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