Method comparison of Particle Enhanced Immunoturbidimetry (PEIT) with High Performance Liquid Chromatography (HPLC) for glycated hemoglobin (HbA1c) analysis.
HbA1c
High performance liquid chromatography (HPLC)
Particle enhanced immunoturbidimetric test (PEIT)
Journal
Clinical diabetes and endocrinology
ISSN: 2055-8260
Titre abrégé: Clin Diabetes Endocrinol
Pays: England
ID NLM: 101669619
Informations de publication
Date de publication:
13 Jun 2021
13 Jun 2021
Historique:
received:
03
11
2020
accepted:
20
05
2021
entrez:
14
6
2021
pubmed:
15
6
2021
medline:
15
6
2021
Statut:
epublish
Résumé
High Performance Liquid Chromatography (HPLC) technique is considered as a gold standard for HbA1c analysis however all laboratories cannot adopt it due to certain limitations. Our aim was to compare Particle Enhanced Immunoturbidimetry (PEIT) method with High Performance Liquid Chromatography (HPLC) for HbA1c analysis. All blood samples were analyzed by HPLC assay on a Bio-Rad D-10 analyzer and PEIT on an Erba XL-200 analyzer. Precision studies were undertaken and Coefficient of Variation (%CV) calculated. Systemic Error (SE), Random Error (RE) and Total Error (TE Our study showed HbA1c analysis by PEIT technique is precise, accurate, rapid and convenient and can be employed as an alternative to HPLC technique in countries where cost is a major problem for diagnosis and treatment.
Sections du résumé
BACKGROUND AND OBJECTIVE
OBJECTIVE
High Performance Liquid Chromatography (HPLC) technique is considered as a gold standard for HbA1c analysis however all laboratories cannot adopt it due to certain limitations. Our aim was to compare Particle Enhanced Immunoturbidimetry (PEIT) method with High Performance Liquid Chromatography (HPLC) for HbA1c analysis.
METHOD
METHODS
All blood samples were analyzed by HPLC assay on a Bio-Rad D-10 analyzer and PEIT on an Erba XL-200 analyzer. Precision studies were undertaken and Coefficient of Variation (%CV) calculated. Systemic Error (SE), Random Error (RE) and Total Error (TE
CONCLUSION
CONCLUSIONS
Our study showed HbA1c analysis by PEIT technique is precise, accurate, rapid and convenient and can be employed as an alternative to HPLC technique in countries where cost is a major problem for diagnosis and treatment.
Identifiants
pubmed: 34120656
doi: 10.1186/s40842-021-00123-w
pii: 10.1186/s40842-021-00123-w
pmc: PMC8201666
doi:
Types de publication
Journal Article
Langues
eng
Pagination
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