RIOK2 phosphorylation by RSK promotes synthesis of the human small ribosomal subunit.
Journal
PLoS genetics
ISSN: 1553-7404
Titre abrégé: PLoS Genet
Pays: United States
ID NLM: 101239074
Informations de publication
Date de publication:
06 2021
06 2021
Historique:
received:
29
04
2021
accepted:
05
05
2021
revised:
24
06
2021
pubmed:
15
6
2021
medline:
28
10
2021
entrez:
14
6
2021
Statut:
epublish
Résumé
Ribosome biogenesis lies at the nexus of various signaling pathways coordinating protein synthesis with cell growth and proliferation. This process is regulated by well-described transcriptional mechanisms, but a growing body of evidence indicates that other levels of regulation exist. Here we show that the Ras/mitogen-activated protein kinase (MAPK) pathway stimulates post-transcriptional stages of human ribosome synthesis. We identify RIOK2, a pre-40S particle assembly factor, as a new target of the MAPK-activated kinase RSK. RIOK2 phosphorylation by RSK stimulates cytoplasmic maturation of late pre-40S particles, which is required for optimal protein synthesis and cell proliferation. RIOK2 phosphorylation facilitates its release from pre-40S particles and its nuclear re-import, prior to completion of small ribosomal subunits. Our results bring a detailed mechanistic link between the Ras/MAPK pathway and the maturation of human pre-40S particles, which opens a hitherto poorly explored area of ribosome biogenesis.
Identifiants
pubmed: 34125833
doi: 10.1371/journal.pgen.1009583
pii: PGENETICS-D-21-00585
pmc: PMC8224940
doi:
Substances chimiques
Protein Serine-Threonine Kinases
EC 2.7.11.1
RIOK2 protein, human
EC 2.7.11.1
Mitogen-Activated Protein Kinases
EC 2.7.11.24
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1009583Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
RNA. 2008 Oct;14(10):2061-73
pubmed: 18755838
Mol Biol Cell. 2012 Jan;23(1):22-35
pubmed: 22072790
Cell. 2012 Oct 26;151(3):671-83
pubmed: 23101633
Science. 2017 Nov 3;358(6363):
pubmed: 29097519
Mol Biol Cell. 2016 Jan 15;27(2):397-409
pubmed: 26582391
RNA Biol. 2012 Feb;9(2):162-74
pubmed: 22418843
Semin Cancer Biol. 2018 Feb;48:53-61
pubmed: 28476656
EMBO J. 2019 Jul 1;38(13):e100278
pubmed: 31268599
EMBO Rep. 2018 Aug;19(8):
pubmed: 29895711
PLoS Genet. 2018 Feb 5;14(2):e1007202
pubmed: 29401457
Mol Cell. 2013 Aug 22;51(4):539-51
pubmed: 23973377
PLoS Biol. 2010 Oct 26;8(10):e1000522
pubmed: 21048991
Trends Biochem Sci. 2017 Aug;42(8):640-654
pubmed: 28579196
Int J Mol Sci. 2019 Jun 03;20(11):
pubmed: 31163577
RNA. 2009 Dec;15(12):2083-104
pubmed: 19850913
Biochim Biophys Acta. 2010 Jun;1803(6):673-83
pubmed: 19879902
J Biol Chem. 1996 Nov 22;271(47):29773-9
pubmed: 8939914
Nat Commun. 2019 Jun 21;10(1):2754
pubmed: 31227701
Nat Struct Mol Biol. 2012 Dec;19(12):1316-23
pubmed: 23104056
Mol Cell. 2001 Nov;8(5):1063-73
pubmed: 11741541
Mol Cell Biol. 2003 Mar;23(6):2083-95
pubmed: 12612080
J Cell Biol. 2009 Jun 29;185(7):1167-80
pubmed: 19564402
PLoS Genet. 2013;9(2):e1003253
pubmed: 23459592
Nat Commun. 2016 Feb 02;7:10336
pubmed: 26831757
Oncogene. 2013 Jun 13;32(24):2917-2926
pubmed: 22797077
Nucleic Acids Res. 2014;42(17):11180-91
pubmed: 25190460
EMBO J. 2003 May 15;22(10):2422-32
pubmed: 12743036
Nat Rev Mol Cell Biol. 2020 Apr;21(4):183-203
pubmed: 31937935
Nucleic Acids Res. 2008 Sep;36(15):5093-101
pubmed: 18676449
Nat Methods. 2017 Jun;14(6):615-620
pubmed: 28417998
Nat Rev Drug Discov. 2019 Jul;18(8):574
pubmed: 31367048
Gene. 2015 Feb 1;556(1):27-34
pubmed: 25447905
J Cell Sci. 2014 Mar 15;127(Pt 6):1242-53
pubmed: 24424021
J Cell Biol. 2010 Sep 6;190(5):853-66
pubmed: 20819938
Nucleic Acids Res. 2017 Jun 20;45(11):6822-6836
pubmed: 28402503
J Cell Biol. 2015 Mar 16;208(6):745-59
pubmed: 25778921
Biochim Biophys Acta. 2005 Dec 30;1754(1-2):14-24
pubmed: 16182620
Biochem J. 2012 Jan 15;441(2):553-69
pubmed: 22187936
Cell Signal. 1992 Nov;4(6):665-74
pubmed: 1489659
Proc Natl Acad Sci U S A. 1994 May 10;91(10):4441-5
pubmed: 8183928
Mol Cell Biol. 2014 Dec;34(24):4474-84
pubmed: 25288641
Wiley Interdiscip Rev RNA. 2019 Jan;10(1):e1516
pubmed: 30406965
Cell Rep. 2015 Dec 29;13(12):2879-91
pubmed: 26711351
Nucleic Acids Res. 2012 Sep 1;40(17):8646-61
pubmed: 22735702
RNA Biol. 2019 Nov;16(11):1633-1642
pubmed: 31390939
Genes Dev. 2003 Jul 15;17(14):1691-702
pubmed: 12865296
Nucleic Acids Res. 2018 Feb 16;46(3):1441-1456
pubmed: 29237037
Cell Rep. 2018 Feb 13;22(7):1923-1934
pubmed: 29444442
J Biol Chem. 2000 Apr 14;275(15):11249-56
pubmed: 10753934
Oncogene. 2014 Jan 23;33(4):474-83
pubmed: 23318442
RNA. 2011 Jan;17(1):189-200
pubmed: 21097556
EMBO J. 2005 Aug 17;24(16):2862-72
pubmed: 16037817
Mol Cell. 2003 Feb;11(2):405-13
pubmed: 12620228
Nucleic Acids Res. 2012 Mar;40(6):2527-39
pubmed: 22121221
J Neurochem. 2011 Nov;119(3):447-59
pubmed: 21838783
Immunity. 2003 Oct;19(4):535-48
pubmed: 14563318
Biochem J. 2012 Oct 1;447(1):159-66
pubmed: 22827337
Elife. 2015 Apr 21;4:
pubmed: 25895666
Nat Commun. 2020 Jan 9;11(1):156
pubmed: 31919354
J Biol Chem. 2007 May 11;282(19):14056-64
pubmed: 17360704
Microbiol Mol Biol Rev. 2011 Mar;75(1):50-83
pubmed: 21372320
J Biol Chem. 2002 Mar 29;277(13):11591-6
pubmed: 11799119
J Mol Cell Cardiol. 2013 Jun;59:139-47
pubmed: 23510923
Science. 2000 May 26;288(5470):1385-9
pubmed: 10827942
Biomolecules. 2018 Oct 24;8(4):
pubmed: 30356013
Nature. 2018 Jun;558(7709):249-253
pubmed: 29875412
Mol Cell Biol. 2010 Jun;30(12):2947-56
pubmed: 20404093
J Cell Mol Med. 2020 Apr;24(8):4494-4509
pubmed: 32125767
J Biol Chem. 2011 Oct 21;286(42):36352-60
pubmed: 21880710
EMBO J. 2006 Aug 23;25(16):3832-42
pubmed: 16888624
RNA Biol. 2019 Feb;16(2):196-210
pubmed: 30638116
Mol Cell Biol. 2001 Jan;21(1):81-7
pubmed: 11113183
Nature. 2006 Jun 1;441(7093):651-5
pubmed: 16738661
Mol Cell. 2006 Mar 3;21(5):629-39
pubmed: 16507361
Trends Biochem Sci. 1999 Nov;24(11):437-40
pubmed: 10542411
Genetics. 2013 Nov;195(3):643-81
pubmed: 24190922
Nat Methods. 2009 Apr;6(4):275-7
pubmed: 19305406
Biochemistry. 2006 Mar 21;45(11):3626-34
pubmed: 16533045
Wiley Interdiscip Rev RNA. 2015 Mar-Apr;6(2):225-42
pubmed: 25346433
J Biol Chem. 2003 Jun 20;278(25):22537-45
pubmed: 12690111
Mol Cell Biol. 1982 Jun;2(6):685-93
pubmed: 14582163
Cell Cycle. 2007 Oct 1;6(19):2323-6
pubmed: 17938580