Could protein content of Urinary Extracellular Vesicles be useful to detect Cirrhosis in Alcoholic Liver Disease?
Biomarkers
/ metabolism
Blotting, Western
/ methods
Cryoelectron Microscopy
/ methods
Early Diagnosis
Extracellular Vesicles
/ metabolism
Humans
Liquid Biopsy
/ methods
Liver Cirrhosis
/ diagnosis
Liver Diseases, Alcoholic
/ complications
Male
Middle Aged
Pilot Projects
Proteomics
/ methods
Reproducibility of Results
Urinalysis
/ methods
Urinary Tract
/ metabolism
alcoholic liver disease (ALD)
biomarkers
cirrhosis
fibrosis
liquid biopsy
urinary extracellular vesicles (uEVs)
Journal
International journal of biological sciences
ISSN: 1449-2288
Titre abrégé: Int J Biol Sci
Pays: Australia
ID NLM: 101235568
Informations de publication
Date de publication:
2021
2021
Historique:
received:
23
02
2021
accepted:
10
04
2021
entrez:
16
6
2021
pubmed:
17
6
2021
medline:
26
3
2022
Statut:
epublish
Résumé
Alcohol abuse has a high impact on the mortality and morbidity related to a great number of diseases and is responsible for the development of alcoholic liver disease (ALD). It remains challenging to detect and evaluate its severity, which is crucial for prognosis. In this work, we studied if urinary EVs (uEVs) could serve in diagnose and evaluate cirrhosis in ALD. To this purpose, uEVs characterization by cryo-electron microscopy (Cryo-EM), Nanoparticle Tracking Analysis (NTA) and Western blotting (WB) was performed in a cohort of 21 controls and 21 cirrhotic patients. Then, proteomics of uEVs was carried out in a second cohort of 6 controls and 8 patients in order to identify new putative biomarkers for cirrhosis in ALD. Interestingly, uEVs concentration, size and protein composition were altered in cirrhotic patients. From a total of 1304 proteins identified in uEVs, 90 of them were found to be altered in cirrhotic patients. The results suggest that uEVs could be considered as a tool and a supplier of new biomarkers for cirrhosis in ALD, whose application would be especially relevant in chronic patients. Yet, further research is necessary to obtain more relevant result in clinical terms.
Identifiants
pubmed: 34131392
doi: 10.7150/ijbs.59725
pii: ijbsv17p1864
pmc: PMC8193259
doi:
Substances chimiques
Biomarkers
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1864-1877Informations de copyright
© The author(s).
Déclaration de conflit d'intérêts
Competing Interests: The authors have declared that no competing interest exists.
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