Identification of H3N2 NA and PB1-F2 genetic variants and their association with disease symptoms during the 2014-15 influenza season.

H3N2 NA PB1-F2 antigenic drift human influenza symptoms

Journal

Virus evolution
ISSN: 2057-1577
Titre abrégé: Virus Evol
Pays: England
ID NLM: 101664675

Informations de publication

Date de publication:
Jan 2021
Historique:
entrez: 16 6 2021
pubmed: 17 6 2021
medline: 17 6 2021
Statut: epublish

Résumé

The 2014-15 influenza season saw the emergence of an H3N2 antigenic drift variant that formed the 3C.2a HA clade. Whole viral genomes were sequenced from nasopharyngeal swabs of ninety-four patients with confirmed influenza A virus infection and primary human nasal epithelial cell cultures used to efficiently isolate H3N2 viruses. The isolates were classified by HA clade and the presence of a new set of co-selected mutations in NA (a glycosylation site, NAg+) and PB1-F2 (H75P). The NA and PB1-F2 mutations were present in a subset of clade 3C.2a viruses (NAg+F2P), which dominated during the subsequent influenza seasons. In human nasal epithelial cell cultures, a virus with the novel NAg+F2P genotype replicated less well compared with a virus with the parental genotype. Retrospective analyses of clinical data showed that NAg+F2P genotype viruses were associated with increased cough and shortness of breath in infected patients.

Identifiants

pubmed: 34131512
doi: 10.1093/ve/veab047
pii: veab047
pmc: PMC8197029
doi:

Types de publication

Journal Article

Langues

eng

Pagination

veab047

Subventions

Organisme : NIAID NIH HHS
ID : HHSN272201400007C
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI007417
Pays : United States

Informations de copyright

© The Author(s) 2021. Published by Oxford University Press.

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Auteurs

Deena R Blumenkrantz (DR)

W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health,Laurel, MD, USA.

Thomas Mehoke (T)

Research and Exploratory Development Department, Johns Hopkins Applied Physics Laboratory, Laurel, MD, USA.

Kathryn Shaw-Saliba (K)

W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health,Laurel, MD, USA.
Department of Medicine, Division of Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Harrison Powell (H)

W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health,Laurel, MD, USA.

Nicholas Wohlgemuth (N)

W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health,Laurel, MD, USA.

Hsuan Liu (H)

W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health,Laurel, MD, USA.

Elizabeth Macias (E)

Epidemiology Laboratory Service, United States Air Force School of Aerospace Medicine, Wright-Patterson Air Force Base, OH, USA.

Jared Evans (J)

Research and Exploratory Development Department, Johns Hopkins Applied Physics Laboratory, Laurel, MD, USA.

Mitra Lewis (M)

Department of Emergency Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Rebecca Medina (R)

Department of Emergency Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Justin Hardick (J)

Department of Emergency Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Lauren M Sauer (LM)

Department of Emergency Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Andrea Dugas (A)

Department of Emergency Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Anna DuVal (A)

Department of Medicine, Division of Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Andrew P Lane (AP)

Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Charlotte Gaydos (C)

Department of Medicine, Division of Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Department of Emergency Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Richard Rothman (R)

Department of Medicine, Division of Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Department of Emergency Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Peter Thielen (P)

Research and Exploratory Development Department, Johns Hopkins Applied Physics Laboratory, Laurel, MD, USA.

Andrew Pekosz (A)

W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health,Laurel, MD, USA.

Classifications MeSH