The nature of cell division forces in epithelial monolayers.
Animals
Animals, Genetically Modified
Cell Communication
Cell Division
Cell Shape
Chromosome Segregation
Computer Simulation
Dogs
Drosophila Proteins
/ genetics
Drosophila melanogaster
/ genetics
Epithelial Cells
/ metabolism
Madin Darby Canine Kidney Cells
Mechanotransduction, Cellular
Microscopy, Confocal
Microscopy, Fluorescence
Models, Biological
Stress, Mechanical
Time Factors
Time-Lapse Imaging
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
02 08 2021
02 08 2021
Historique:
received:
18
11
2020
revised:
05
04
2021
accepted:
19
05
2021
entrez:
16
6
2021
pubmed:
17
6
2021
medline:
21
10
2021
Statut:
ppublish
Résumé
Epithelial cells undergo striking morphological changes during division to ensure proper segregation of genetic and cytoplasmic materials. These morphological changes occur despite dividing cells being mechanically restricted by neighboring cells, indicating the need for extracellular force generation. Beyond driving cell division itself, forces associated with division have been implicated in tissue-scale processes, including development, tissue growth, migration, and epidermal stratification. While forces generated by mitotic rounding are well understood, forces generated after rounding remain unknown. Here, we identify two distinct stages of division force generation that follow rounding: (1) Protrusive forces along the division axis that drive division elongation, and (2) outward forces that facilitate postdivision spreading. Cytokinetic ring contraction of the dividing cell, but not activity of neighboring cells, generates extracellular forces that propel division elongation and contribute to chromosome segregation. Forces from division elongation are observed in epithelia across many model organisms. Thus, division elongation forces represent a universal mechanism that powers cell division in confining epithelia.
Identifiants
pubmed: 34132746
pii: 212389
doi: 10.1083/jcb.202011106
pmc: PMC8240854
pii:
doi:
Substances chimiques
Drosophila Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM116000
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM126256
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007287
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007790
Pays : United States
Informations de copyright
© 2021 Gupta et al.
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