Longitudinal high-resolution imaging through a flexible intravital imaging window.
Journal
Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440
Informations de publication
Date de publication:
Jun 2021
Jun 2021
Historique:
received:
27
01
2021
accepted:
30
04
2021
entrez:
17
6
2021
pubmed:
18
6
2021
medline:
18
6
2021
Statut:
epublish
Résumé
Intravital microscopy (IVM) is a powerful technique that enables imaging of internal tissues at (sub)cellular resolutions in living animals. Here, we present a silicone-based imaging window consisting of a fully flexible, sutureless design that is ideally suited for long-term, longitudinal IVM of growing tissues and tumors. Crucially, we show that this window, without any customization, is suitable for numerous anatomical locations in mice using a rapid and standardized implantation procedure. This low-cost device represents a substantial technological and performance advance that facilitates intravital imaging in diverse contexts in higher organisms, opening previously unattainable avenues for in vivo imaging of soft and fragile tissues.
Identifiants
pubmed: 34134982
pii: 7/25/eabg7663
doi: 10.1126/sciadv.abg7663
pmc: PMC8208712
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY).
Références
Nat Methods. 2008 Dec;5(12):1019-21
pubmed: 18997781
J Orthop Res. 2005 Sep;23(5):1073-82
pubmed: 15890486
Nat Protoc. 2011 Aug 18;6(9):1355-66
pubmed: 21886101
Cancer Sci. 2016 May;107(5):644-52
pubmed: 26931406
Nature. 2017 Sep 7;549(7670):96-100
pubmed: 28854174
Genesis. 2007 Sep;45(9):593-605
pubmed: 17868096
Nat Protoc. 2013 Mar;8(3):583-94
pubmed: 23429719
Am J Pathol. 1993 Oct;143(4):1055-62
pubmed: 7692730
Cell Stem Cell. 2016 Feb 4;18(2):243-52
pubmed: 26686466
Sci Transl Med. 2012 Oct 31;4(158):158ra145
pubmed: 23115354
Nat Commun. 2019 Dec 11;10(1):5647
pubmed: 31827103
Front Cell Dev Biol. 2020 Mar 31;8:203
pubmed: 32296702
Nat Commun. 2019 Apr 2;10(1):1500
pubmed: 30940809
Sci Rep. 2016 Jun 10;6:27818
pubmed: 27283875
Intravital. 2016 Jan 13;5(1):e1125562
pubmed: 28243517
Nat Methods. 2005 Dec;2(12):932-40
pubmed: 16299478
Cell. 2011 Nov 23;147(5):983-91
pubmed: 22118457
J Cell Sci. 2018 Mar 6;131(5):
pubmed: 29511095
J Cell Sci. 2017 Jan 1;130(1):23-38
pubmed: 27505891
Cell Rep. 2020 Mar 10;30(10):3195-3206.e7
pubmed: 32160529
Intravital. 2016 Apr 8;5(2):e1156272
pubmed: 28243520
Cell Immunol. 2001 Dec 15;214(2):110-22
pubmed: 12088410
Nat Methods. 2018 Jan;15(1):73-80
pubmed: 29176592
Nat Protoc. 2009;4(8):1128-44
pubmed: 19617885
Development. 2011 Sep;138(17):3625-37
pubmed: 21828091
J Vis Exp. 2014 Apr 12;(86):
pubmed: 24747942
Intravital. 2014 Aug 11;3(2):e29917
pubmed: 28243510
Annu Rev Cell Dev Biol. 2015;31:373-97
pubmed: 26407212
J Exp Med. 2001 Oct 15;194(8):1151-64
pubmed: 11602643
Cold Spring Harb Protoc. 2011 Feb 01;2011(2):pdb.prot5563
pubmed: 21285263
Nephron Exp Nephrol. 2006;103(2):e33-40
pubmed: 16543762
Stem Cells. 2013 Mar;31(3):602-6
pubmed: 23225641
Science. 2020 Apr 10;368(6487):181-186
pubmed: 32273467
Cytometry A. 2020 May;97(5):448-457
pubmed: 31889408