Engorgement of Rhipicephalus haemaphysaloides ticks blocked by silencing a protein inhibitor of apoptosis.
IAP
RNA interference
Rhipicephalus haemaphysaloides
Salivary glands
Journal
Experimental & applied acarology
ISSN: 1572-9702
Titre abrégé: Exp Appl Acarol
Pays: Netherlands
ID NLM: 8507436
Informations de publication
Date de publication:
Jul 2021
Jul 2021
Historique:
received:
17
03
2021
accepted:
14
06
2021
pubmed:
18
6
2021
medline:
8
7
2021
entrez:
17
6
2021
Statut:
ppublish
Résumé
Inhibitors of apoptosis (IAPs) are regulators of cell death and may play a role in the salivary glands of ticks during blood-feeding. We cloned the open reading frame (ORF) sequence of the IAP gene in Rhipicephalus haemaphysaloides (RhIAP). The RhIAP ORF of 1887 bp encodes a predicted protein of 607 amino acids, which contains three baculovirus IAP repeat domains and a RING finger motif. A real-time PCR assay showed that RhIAP mRNA was expressed in all the tick developmental stages (eggs, larvae, nymphs, and adults) and in all tissues examined (midgut, ovary, salivary glands, fat body, and hemolymph). Western blot showed that the protein level of RhIAP in salivary glands increased during tick blood-feeding and decreased towards the end of tick engorgement. RhIAP gene silencing in vitro experiments with salivary glands demonstrated that RhIAP could be effectively knocked down within 48 h after dsRNA treatment, and as a consequence, salivary glands displayed apoptotic morphology. RhIAP gene silencing also inhibited tick blood-feeding and decreased the engorgement rate. These data suggest that RhIAP might be a suitable RNAi target for tick control.
Identifiants
pubmed: 34136982
doi: 10.1007/s10493-021-00637-z
pii: 10.1007/s10493-021-00637-z
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
623-636Références
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