The effects of testosterone on gene expression of cell-adhesion molecules and scaffolding proteins: The role of sex in early development.
brain development
sex differences
synaptic proteins
testosterone
Journal
Andrologia
ISSN: 1439-0272
Titre abrégé: Andrologia
Pays: Germany
ID NLM: 0423506
Informations de publication
Date de publication:
Oct 2021
Oct 2021
Historique:
revised:
28
05
2021
received:
10
03
2021
accepted:
02
06
2021
pubmed:
18
6
2021
medline:
14
9
2021
entrez:
17
6
2021
Statut:
ppublish
Résumé
Sex-specific differences in brain plasticity appear to be organised by testosterone, which is particularly important during the early stages of development. The main purpose of the present study was to examine the sex differences in mRNA and protein levels of selected cell-adhesion molecules and scaffolding proteins on postnatal days 5 (P5) and 9 (P9) in the rat hippocampus, as well as evaluate the effects of testosterone treatment (100 nM, 48 hr) on synaptic proteins in SH-SY5Y (neuron-like) and U-87MG (astrocyte-like) cells. The gene expression levels of Neuroligin 3 and 'SH3 and multiple ankyrin repeat domains protein' 1 and 3 (SHANK1 and SHANK3) were significantly lower in males compared to females at P5. At P9, a similar significant trend towards a decrease in mRNA expression and protein levels of SHANK3 was found in males. Testosterone treatment induced a significant decrease of Neuroligin 1-3 mRNA expression in both SH-SY5Y and U-87MG cells. SHANK1 and SHANK3 mRNA levels significantly decreased in U-87MG cells response to testosterone presence. The presented results demonstrate that the association of selected postsynaptic cell-adhesion molecules and scaffolding proteins is sex-related. Testosterone appears to be particularly involved in the developmental mechanisms related to neuroplasticity.
Substances chimiques
RNA, Messenger
0
Testosterone
3XMK78S47O
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e14153Subventions
Organisme : Grant Agency of the Ministry of Education and the Slovak Academy of Sciences
ID : VEGA 2/0155/20
Informations de copyright
© 2021 Wiley-VCH GmbH.
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