Feasibility of collecting and processing of COVID-19 convalescent plasma for treatment of COVID-19 in Uganda.
Adolescent
Adult
Aged
Antibodies, Viral
/ blood
Blood Donors
Blood Specimen Collection
/ methods
COVID-19
/ therapy
Convalescence
Cross-Sectional Studies
Feasibility Studies
Female
Humans
Immunization, Passive
/ methods
Male
Middle Aged
SARS-CoV-2
/ immunology
Uganda
Young Adult
COVID-19 Serotherapy
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2021
2021
Historique:
received:
24
10
2020
accepted:
06
03
2021
entrez:
17
6
2021
pubmed:
18
6
2021
medline:
13
7
2021
Statut:
epublish
Résumé
Evidence that supports the use of COVID-19 convalescent plasma (CCP) for treatment of COVID-19 is increasingly emerging. However, very few African countries have undertaken the collection and processing of CCP. The aim of this study was to assess the feasibility of collecting and processing of CCP, in preparation for a randomized clinical trial of CCP for treatment of COVID-19 in Uganda. In a cross-sectional study, persons with documented evidence of recovery from COVID-19 in Uganda were contacted and screened for blood donation via telephone calls. Those found eligible were asked to come to the blood donation centre for further screening and consent. Whole blood collection was undertaken from which plasma was processed. Plasma was tested for transfusion transmissible infections (TTIs) and anti-SARS CoV-2 antibody titers. SARS-CoV-2 testing was also done on nasopharyngeal swabs from the donors. 192 participants were contacted of whom 179 (93.2%) were eligible to donate. Of the 179 eligible, 23 (12.8%) were not willing to donate and reasons given included: having no time 7(30.4%), fear of being retained at the COVID-19 treatment center 10 (43.5%), fear of stigma in the community 1 (4.3%), phobia for donating blood 1 (4.3%), religious issues 1 (4.4%), lack of interest 2 (8.7%) and transport challenges 1 (4.3%). The median age was 30 years and females accounted for 3.7% of the donors. A total of 30 (18.5%) donors tested positive for different TTIs. Antibody titer testing demonstrated titers of more than 1:320 for all the 72 samples tested. Age greater than 46 years and female gender were associated with higher titers though not statistically significant. CCP collection and processing is possible in Uganda. However, concerns about stigma and lack of time, interest or transport need to be addressed in order to maximize donations.
Identifiants
pubmed: 34138909
doi: 10.1371/journal.pone.0252306
pii: PONE-D-20-33479
pmc: PMC8211202
doi:
Substances chimiques
Antibodies, Viral
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0252306Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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