Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (2): impacts of post-partum negative energy balance on stromal, glandular and luminal epithelial cells.
Cell type-specific
Endometrium
Inflammation
LCM
Negative energy balance
RNA-seq
Transcriptome
Journal
BMC genomics
ISSN: 1471-2164
Titre abrégé: BMC Genomics
Pays: England
ID NLM: 100965258
Informations de publication
Date de publication:
18 Jun 2021
18 Jun 2021
Historique:
received:
18
01
2021
accepted:
13
05
2021
entrez:
18
6
2021
pubmed:
19
6
2021
medline:
22
6
2021
Statut:
epublish
Résumé
In post-partum dairy cows, the energy needs to satisfy high milk production induces a status of more or less pronounced Negative Energy Balance (NEB). NEB associated with fat mobilization impairs reproductive function. In a companion paper, we described constitutive gene expression in the three main endometrial cell types (stromal, glandular and luminal epithelial cells) isolated by laser capture micro-dissection (LCM) showing the specificities of their transcriptomic profiles. This study investigates the specific impact of NEB on gene expression in these cells around 80 days after parturition at day 15 of the oestrus cycle and describes their specific response to NEB. Following the description of their constitutive expression, the transcriptome profiles obtained by RNA sequencing of the three cells types revealed that differences related to the severity of NEB altered mainly specific patterns of expression related to individual cell types. Number of differentially expressed genes between severe NEB (SNEB) and mild NEB (MNEB) cows was higher in ST than in LE and GE, respectively. SNEB was associated with differential expression of genes coding for proteins involved in metabolic processes and embryo-maternal interactions in ST. Under-expression of genes encoding proteins with functions related to cell structure was found in GE whereas genes encoding proteins participating in pro-inflammatory pathways were over-expressed. Genes associated to adaptive immunity were under-expressed in LE. The severity of NEB after calving is associated with changes in gene expression around 80 days after parturition corresponding to the time of breeding. Specific alterations in GEs are associated with activation of pro-inflammatory mechanisms. Concomitantly, changes in the expression of genes encoding proteins involved in cell interactions and maternal recognition of pregnancy takes place in ST. The combination of these effects possibly altering the uterine environment and embryo maternal interactions may negatively influence the establishment of pregnancy.
Sections du résumé
BACKGROUND
BACKGROUND
In post-partum dairy cows, the energy needs to satisfy high milk production induces a status of more or less pronounced Negative Energy Balance (NEB). NEB associated with fat mobilization impairs reproductive function. In a companion paper, we described constitutive gene expression in the three main endometrial cell types (stromal, glandular and luminal epithelial cells) isolated by laser capture micro-dissection (LCM) showing the specificities of their transcriptomic profiles. This study investigates the specific impact of NEB on gene expression in these cells around 80 days after parturition at day 15 of the oestrus cycle and describes their specific response to NEB.
RESULTS
RESULTS
Following the description of their constitutive expression, the transcriptome profiles obtained by RNA sequencing of the three cells types revealed that differences related to the severity of NEB altered mainly specific patterns of expression related to individual cell types. Number of differentially expressed genes between severe NEB (SNEB) and mild NEB (MNEB) cows was higher in ST than in LE and GE, respectively. SNEB was associated with differential expression of genes coding for proteins involved in metabolic processes and embryo-maternal interactions in ST. Under-expression of genes encoding proteins with functions related to cell structure was found in GE whereas genes encoding proteins participating in pro-inflammatory pathways were over-expressed. Genes associated to adaptive immunity were under-expressed in LE.
CONCLUSION
CONCLUSIONS
The severity of NEB after calving is associated with changes in gene expression around 80 days after parturition corresponding to the time of breeding. Specific alterations in GEs are associated with activation of pro-inflammatory mechanisms. Concomitantly, changes in the expression of genes encoding proteins involved in cell interactions and maternal recognition of pregnancy takes place in ST. The combination of these effects possibly altering the uterine environment and embryo maternal interactions may negatively influence the establishment of pregnancy.
Identifiants
pubmed: 34139988
doi: 10.1186/s12864-021-07713-z
pii: 10.1186/s12864-021-07713-z
pmc: PMC8212477
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
450Subventions
Organisme : Seventh Framework Programme
ID : FP7-KBBE-2012-6 Prolific and Grant agreement number 311776
Références
Lipids Health Dis. 2017 Apr 28;16(1):83
pubmed: 28454542
J Reprod Dev. 2012;58(2):189-95
pubmed: 22738902
Res Vet Sci. 2009 Aug;87(1):59-63
pubmed: 19181355
F1000Res. 2015 Dec 30;4:1521
pubmed: 26925227
Anim Reprod Sci. 2014 Jan 30;144(3-4):60-71
pubmed: 24378117
Mech Dev. 2002 Feb;111(1-2):99-113
pubmed: 11804782
Reprod Fertil Dev. 2017 Feb;29(2):274-282
pubmed: 26304020
Reprod Fertil Dev. 2012;25(1):48-61
pubmed: 23244828
J Biol Chem. 1982 Jun 25;257(12):6886-97
pubmed: 6211438
J Reprod Dev. 2010 Jan;56 Suppl:S15-21
pubmed: 20629212
Cell. 2002 Apr;109 Suppl:S121-31
pubmed: 11983158
Biol Reprod. 2001 Nov;65(5):1311-23
pubmed: 11673245
J Anim Sci. 1981 Sep;53(3):742-8
pubmed: 7319952
Theriogenology. 2001 Dec 1;56(9):1435-50
pubmed: 11768809
Biol Reprod. 2016 Oct;95(4):88
pubmed: 27535962
F1000Res. 2015 Oct 14;4:1070
pubmed: 26674615
J Dairy Sci. 2012 Oct;95(10):5645-56
pubmed: 22863093
Biol Reprod. 2018 Jul 1;99(1):225-241
pubmed: 29462279
Reproduction. 2008 Jan;135(1):63-75
pubmed: 18159084
Theriogenology. 2006 May;65(8):1516-30
pubmed: 16226305
Physiol Genomics. 2016 Apr;48(4):306-19
pubmed: 26850042
J Dairy Sci. 2013 Feb;96(2):918-35
pubmed: 23261380
Theriogenology. 2004 Jul;62(1-2):9-23
pubmed: 15159097
J Dairy Sci. 1989 Mar;72(3):767-83
pubmed: 2654227
Nucleic Acids Res. 2002 Jan 1;30(1):207-10
pubmed: 11752295
Asian-Australas J Anim Sci. 2013 Sep;26(9):1255-61
pubmed: 25049907
J Transl Med. 2016 Oct 26;14(1):303
pubmed: 27782833
Stem Cells. 2001;19(5):378-87
pubmed: 11553846
Endocrinology. 1994 Feb;134(2):521-8
pubmed: 8299552
BMC Cell Biol. 2010 Dec 06;11:95
pubmed: 21134253
Genome Biol. 2014;15(12):550
pubmed: 25516281
J Anim Sci. 2004;82 E-Suppl:E4-13
pubmed: 15471813
Physiol Genomics. 2012 Jan 18;44(1):47-58
pubmed: 22045914
Vet Q. 2005 Sep;27(3):117-26
pubmed: 16238111
Biol Reprod. 2000 Aug;63(2):508-12
pubmed: 10906057
Semin Reprod Med. 2007 Jan;25(1):52-9
pubmed: 17205423
Biol Reprod. 2017 Sep 1;97(3):413-425
pubmed: 29024972
Trends Endocrinol Metab. 2011 Jan;22(1):16-23
pubmed: 20888253
J Endocrinol. 1998 Jun;157(3):405-14
pubmed: 9691973
J Dairy Sci. 2012 Oct;95(10):5657-75
pubmed: 22884349
Biol Reprod. 2002 Jul;67(1):161-9
pubmed: 12080013
Reproduction. 2013 Apr 29;145(5):527-39
pubmed: 23533291
Endocrinology. 2006 Jul;147(7):3375-90
pubmed: 16627586
Endocrinology. 2011 Nov;152(11):4158-70
pubmed: 21862610
Soc Reprod Fertil Suppl. 2007;64:379-96
pubmed: 17491160
Hum Reprod Update. 2006 Nov-Dec;12(6):731-46
pubmed: 16982667
Reproduction. 2014 Dec;148(6):545-57
pubmed: 25187621
Placenta. 2002 Sep-Oct;23(8-9):672-84
pubmed: 12361686
Reprod Sci. 2014 Jun;21(6):733-42
pubmed: 24336674
Am J Physiol Endocrinol Metab. 2013 Apr 1;304(7):E770-9
pubmed: 23423172
Biol Reprod. 2001 Jun;64(6):1608-13
pubmed: 11369585
Physiol Rev. 2002 Jan;82(1):131-85
pubmed: 11773611
Proteomics. 2016 May;16(10):1474-85
pubmed: 27030549
Nucleic Acids Res. 2019 Jan 8;47(D1):D607-D613
pubmed: 30476243
Int J Dev Biol. 2014;58(2-4):107-16
pubmed: 25023676
Reprod Fertil Dev. 2018 Nov;30(12):1770-1784
pubmed: 30086824
Reproduction. 2009 Feb;137(2):259-69
pubmed: 18996977
Physiol Genomics. 2009 Sep 9;39(1):1-13
pubmed: 19567787
Biol Reprod. 2015 Apr;92(4):106
pubmed: 25695723
Reprod Fertil Dev. 2019 Mar;31(3):496-508
pubmed: 30253121
Domest Anim Endocrinol. 2008 May;34(4):391-402
pubmed: 18258405
Mol Hum Reprod. 2013 Sep;19(9):547-58
pubmed: 23619340
Reproduction. 2011 Feb;141(2):269-81
pubmed: 21123519
J Dairy Sci. 1990 Oct;73(10):2749-58
pubmed: 2283405
PLoS One. 2011;6(8):e23183
pubmed: 21858021
Am J Reprod Immunol. 2017 Jun;77(6):
pubmed: 28185389
J Nutr. 2008 Jun;138(6):1019-24
pubmed: 18492828
Anim Reprod Sci. 2004 Apr;81(3-4):225-35
pubmed: 14998649
Mol Hum Reprod. 2010 Mar;16(3):135-52
pubmed: 19880575
Cell Mol Immunol. 2014 Sep;11(5):438-48
pubmed: 25109684
Nat Methods. 2017 Apr;14(4):417-419
pubmed: 28263959
J Endocrinol. 1968 Jul;41(3):363-71
pubmed: 5711112
Biol Reprod. 1999 Nov;61(5):1267-74
pubmed: 10529273
Vet Clin North Am Food Anim Pract. 2004 Nov;20(3):447-70
pubmed: 15471620
J Reprod Fertil. 1988 May;83(1):239-48
pubmed: 3165129
J Endocrinol. 2003 Feb;176(2):205-17
pubmed: 12553869
Am J Reprod Immunol. 2005 Feb;53(2):65-76
pubmed: 15790340
Theriogenology. 2017 Mar 1;90:276-283
pubmed: 28166980
Immunology. 2004 Jul;112(3):428-36
pubmed: 15196211
J Dairy Sci. 2018 May;101(5):3722-3741
pubmed: 29501340
Fertil Steril. 1994 Sep;62(3):497-506
pubmed: 8062944