Magnetic Resonance Imaging of Protease-Mediated Lung Tissue Inflammation and Injury.
Journal
ACS omega
ISSN: 2470-1343
Titre abrégé: ACS Omega
Pays: United States
ID NLM: 101691658
Informations de publication
Date de publication:
15 Jun 2021
15 Jun 2021
Historique:
received:
03
03
2021
accepted:
18
05
2021
entrez:
21
6
2021
pubmed:
22
6
2021
medline:
22
6
2021
Statut:
epublish
Résumé
Pulmonary inflammation usually involves strong neutrophil recruitment with a marked release of proteases such as neutrophil elastase (NE). Noninvasive in vivo assessment of unregulated elastase activity in the lungs would provide a valuable diagnostic tool. Here, it is proposed to use Overhauser-enhanced magnetic resonance imaging (OMRI) in mice where inflammation was induced by the instillation of lipopolysaccharide (LPS). OMRI contrast in the lungs was generated by a dedicated NE free radical substrate. The free radical decayed more rapidly in LPS-treated mouse lungs than in control mice, indicating the occurrence of increased proteolysis under inflammation. Preclinical detection of abnormal proteolysis opens the way for new diagnosis modality and antiprotease testing in vivo.
Identifiants
pubmed: 34151082
doi: 10.1021/acsomega.1c01150
pmc: PMC8209802
doi:
Types de publication
Journal Article
Langues
eng
Pagination
15012-15016Informations de copyright
© 2021 The Authors. Published by American Chemical Society.
Déclaration de conflit d'intérêts
The authors declare no competing financial interest.
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