Microtubule destabilization is a critical checkpoint of chemotaxis and transendothelial migration in melanoma cells but not in T cells.
Animals
Cell Line, Tumor
Cell Movement
/ drug effects
Chemotaxis
/ drug effects
Macrolides
/ pharmacology
Male
Melanoma
/ pathology
Mice
Mice, Inbred C57BL
Microtubules
/ drug effects
Pseudopodia
/ drug effects
T-Lymphocytes
/ drug effects
Transendothelial and Transepithelial Migration
/ drug effects
Cytoskeleton
cancer
metastasis
motility
taxol
Journal
Cell adhesion & migration
ISSN: 1933-6926
Titre abrégé: Cell Adh Migr
Pays: United States
ID NLM: 101469464
Informations de publication
Date de publication:
12 2021
12 2021
Historique:
entrez:
21
6
2021
pubmed:
22
6
2021
medline:
15
12
2021
Statut:
ppublish
Résumé
Microtubules (MTs) control cell shape and intracellular cargo transport. The role of MT turnover in the migration of slow-moving cells through endothelial barriers remains unclear. To irreversibly interfere with MT disassembly, we have used the MT-stabilizing agent zampanolide (ZMP) in Β16F10 melanoma as amodel of slow-moving cells. ZMP-treated B16 cells failed to follow chemotactic gradients across rigid confinements and could not generate stable sub-endothelial pseudopodia under endothelial monolayers. In vivo, ZMP-treated Β16 cells failed to extravasate though lung capillaries. In contrast to melanoma cells, the chemotaxis and transendothelial migration of ZMP-treated Tcells were largely conserved. This is afirst demonstration that MT disassembly is akey checkpoint in the directional migration of cancer cells but not of lymphocytes.
Identifiants
pubmed: 34152257
doi: 10.1080/19336918.2021.1934958
pmc: PMC8218694
doi:
Substances chimiques
Macrolides
0
zampanolide
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
166-179Références
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